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Identification of a fibronectin-binding protein of Treponema lecithinolyticum by two-dimensional gel electrophoresis and ligand binding assay

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Abstract:

Treponema lecithinolyticum is associated with periodontitis and endodontic infections. As a critical early step in the infection process, fibronectin-binding protein (Fbp) is known to be involved in the adhesion of bacteria to cell surfaces for colonization and, hence, is considered to be a virulence factor. In this study, we identified an Fbp from the T.lecithinolyticum cell surface with a molecular mass of about 52kDa by using 2-dimensional gel electrophoresis followed by a ligand binding assay. As T.lecithinolyticum is capable of binding to soluble and immobilized fibronectin, this Fbp may contribute to bacterial attachment to host cells.

Treponema lecithinolyticum est associée aux infections péridontales et endodontales. La protéine liant la fibronectine Fbp est reconnue pour son implication dans l’adhésion des bactéries aux surfaces cellulaires lors de la colonisation en tant qu’étape précoce critique du processus infectieux et ce faisant, elle constitue un facteur de virulence. Dans cette étude, nous avons identifié une Fbp à la surface de T.lecithinolyticum d’une masse moléculaire de 52 kDa environ par électrophorèse sur gel en deux dimensions et par un essai de liaison de la fibronectine. Puisque que T. lecithinolyticum est capable de lier la fibronectine soluble et immobilisée, cette Fbp pourrait contribuer à l’attachement des bactéries aux cellules hôtes.

Document Type: Research Article

Publication date: October 1, 2007

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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