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Polar vineyard pruning extracts increase the activity of the main ligninolytic enzymes in Lentinula edodes cultures

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Lentinula edodes is considered an alternative recycling agent for agricultural wastes, and there have been several studies to understand the relationship between its growth and ligninolytic activity. We tested the effect of wood from viticulture pruning, extracted with solvents of differing polarity, on the biomass production and activity pattern of ligninolytic enzymes. The analysis was done by measuring the mycelial dry mass and enzyme activity of liquid growth medium during the culture of L. edodes, adding either single extracts or a combination of extracts. Polar extracts enhanced mycelial production, and the activity patterns of lignin peroxidase, manganese peroxidase, aryl alcohol oxidase, and laccase were comparable to their activities predicted by ligninolysis models proposed for other fungi. We conclude that the polar extracts could be useful for enhancing fungal biomass production and for modifying lignin degradation because the regulation of ligninolytic enzyme activity is differentially influenced by the polarity of the extract.

Lentinula edodes est considéré comme un agent de recyclage alternatif des déchets agricoles et plusieurs études ont été réalisées afin de comprendre sa croissance en lien avec son activité ligninolytique. Nous avons testé l’effet de bois d’élagage viticole, extrait avec des solvants de polarités différentes, sur la production de la biomasse et le patron d’activité des enzymes ligninolytiques. L’analyse a été faite en quantifiant le poids sec de mycélium et l’activité enzymatique en culture liquide de L. edodes après un ajout d’extraits simples ou combinés. Les extraits polaires ont augmenté la production de mycélium, et les patrons d’activité de la lignine peroxydase, de la manganèse peroxydase, de l’aryl alcool oxydase et de la laccase étaient comparables aux activités prédites par des modèles de ligninolyse proposés chez d’autres champignons. Nous concluons que les extraits polaires pourraient être utiles pour augmenter la production de biomasse fongique et pour modifier la dégradation de lignine parce que la régulation de l’activité enzymatique ligninolytique est influencée de façon différentielle par la polarité de l’extrait.

Document Type: Research Article

Publication date: October 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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