Preliminary investigations on inducing salt tolerance in maize through inoculation with rhizobacteria containing ACC deaminase activity

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Abstract:

Twenty rhizobacterial strains containing 1-aminocyclopropane-1-carboxylate deaminase were isolated from the rhizosphere of salt-affected maize fields. They were screened for their growth-promoting activities under axenic conditions at 1, 4, 8, and 12dS·m–1 salinity levels. Based upon the data of the axenic study, the 6 most effective strains were selected to conduct pot trials in the wire house. Besides one original salinity level (1.6dS·m–1), 3 other salinity levels (4, 8, and 12dS·m–1) were maintained in pots and maize seeds inoculated with selected strains of plant growth-promoting rhizobacteria, as well as uninoculated controls were sown. Results showed that the increase in salinity level decreased the growth of maize seedlings. However, inoculation with rhizobacterial strains reduced this depression effect and improved the growth and yield at all the salinity levels tested. Selected strains significantly increased plant height, root length, total biomass, cob mass, and grain yield up to 82%, 93%, 51%, 40%, and 50%, respectively, over respective uninoculated controls at the electrical conductivity of 12dS·m–1. Among various plant growth-promoting rhizobacterial strains, S5 (Pseudomonas syringae), S14 (Enterobacter aerogenes), and S20 (Pseudomonas fluorescens) were the most effective strains for promoting the growth and yield of maize, even at high salt stress. The relatively better salt tolerance of inoculated plants was associated with a high K+/Na+ ratio as well as high relative water and chlorophyll and low proline contents.

Vingt souches de rhizobactéries exprimant l’ACC désaminase ont été isolées de la rhizosphère de champs de maïs exposés au sel. Elles ont été évaluées relativement à leur activité promotrice de la croissance sous des conditions axéniques à des niveaux de salinité de 1, 4, 8 et 12dS·m–1. Selon les résultats de l’étude en conditions axéniques, les 6 souches les plus efficaces ont été sélectionnées pour réaliser des essais en pots. Outre le niveau original de 1,6dS·m–1, 3 niveaux de salinité, soit 4, 8 et 12dS·m–1, ont été maintenus dans les pots, et des grains de maïs inoculés ou non (contrôles) avec les souches de rhizobactéries favorisant la croissance des plantes (RFCP) sélectionnées ont été semés dans les pots. Nos résultats ont montré que l’augmentation de la salinité diminuait la croissance des pousses de maïs. Cependant, l’inoculation des souches de rhizobactéries a réduit cet effet inhibiteur et a amélioré la croissance et la récolte dans toutes les conditions de salinité testées. Les souches sélectionnées ont augmenté significativement la hauteur des plants, la longueur des racines, la biomasse totale, le poids de l’épi et le rendement de 82%, 93%, 51%, 40% et 50% respectivement, par rapport aux contrôles non inoculés dans des conditions de salinité de 12dS·m–1. Parmi les différentes souches de RFCP, S5 (Pseudomonas syringae), S14 (Enterobacter aerogenes) et S20 (Pseudomonas fluorescence) étaient les plus efficaces à promouvoir la croissance et le rendement du maïs, même sous un stress de haute salinité. Une meilleure tolérance relative au sel des plants inoculés était associée à un rapport K+/Na+ élevé, de même qu’au contenu relatif en eau, en chlorophylle et faible en proline.

Document Type: Research Article

Publication date: October 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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