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Phylogenetic analysis of norovirus isolates involved in some Canadian gastroenteritis outbreaks in 2004 and 2005

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Noroviruses are recognized as the most common cause of nonbacterial gastroenteritis worldwide. In this study, we investigated the molecular epidemiology of noroviral isolates in Canada from 2004 to 2005 by sequencing the RNA polymerase gene and capsid N-terminal/shell (N/S) domain. Norovirus genogroups I and II were thus found to have co-circulated in Canada during the studied period, with a higher incidence of genogroup II (95.7%). The GII-4 or Lordsdale subgroup was the predominant genotype, suggesting that norovirus genogroup II is the major cause of viral gastroenteritis in Canada, as it is in many other countries. Phylogenetic analyses of the RNA polymerase gene and the capsid N/S domain indicated different genotypes for 2 strains, suggesting probable genetic recombination. Sequencing of the norovirus polymerase gene may reflect actual classification but should be supported by sequence information obtained from the capsid gene.

Les norovirus sont reconnus comme la cause la plus fréquente des gastroentérites non bactériennes à travers le monde. Dans cette étude, nous avons examiné l’épidémiologie moléculaire d’isolats prélevés au Canada entre 2004 et 2005 par séquençage des gènes de l’ARN polymérase et du domaine N/S de la capside. Nous avons trouvé que les génogroupes I et II de norovirus ont co-circulé au Canada durant la période étudiée, le génogroupe II présentant la plus forte incidence (95,7%). Le sous-groupe GII-4 ou Lordsdale était le génotype prédominant, suggérant que le génogroupe II de norovirus soit la cause principale de gastroentérite virale au Canada, comme dans plusieurs autres pays. Les analyses phylogéniques des gènes de l’ARN polymérase et du domaine N/S de la capside ont révélé la présence de différents génotypes chez 2 souches, suggérant une probable recombinaison génétique. La séquence du gène de la polymérase des norovirus refléter leur classification actuelle, mais elle doit être appuyée par des informations de séquence du gène de la capside.

Document Type: Research Article

Publication date: October 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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