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Bioprospecting highly diverse endophytic Pestalotiopsis spp. with antibacterial properties from Maytenus ilicifolia, a medicinal plant from Brazil

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Abstract:

Thirteen endophytic fungal strains of the genus Pestalotiopsis were isolated from the medicinal plant Maytenus ilicifolia Mart. ex. Reiss (commonly known as “espinheira santa”) and their antimicrobial properties were investigated. Two isolates were successful in inhibiting the growth of the tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA)) using the technique of bioautographic thin-layer chromatography (TLC) agar overlay assay. An analysis based on a polyphasic approach integrating taxonomic information, morphological traits, RAPD markers, and the sequencing of the ITS1–5.8S–ITS2 of the rDNA led to the assignment of the isolates as belonging to the species Pestalotiopsis microspora, Pestalotiopsis vismiae, and Pestalotiopsis leucothoes. Therefore, the present study presents a new approach to the study of endophytic fungi of the genus Pestalotiopsis.

Treize souches d’endophytes fongiques du genre Pestalotiopsis ont été isolées de la plante médicinale Maytenus ilicifolia Mart. Ex. Reiss (communément appelée «espinheira santa», et testées relativement à leurs propriétés antimicrobiennes. Deux isolats ont pu inhiber la croissance de divers microorganismes (Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Staphylococcus aureus et le Staphylococcus aureus résistant à la méthicilline (SARM)), tel que détecté par un essai bio-autographique en CCM/couche supérieure de gélose. Une analyse basée sur une approche polyphasique intégrant une information taxonomique, des traits morphologiques, des marqueurs RAPD et le séquençage des régions ITS1–5.8S–ITS2 de l’ADNr a permis d’attribuer les isolats aux espèces Pestalotiopsis microspora, Pestalotiopsis vismiae et Pestalotiopsis leucothoes. Ainsi, la présente étude présente une nouvelle approche pour étudier les endophytes fongiques du genre Pestalotiopsis.

Document Type: Research Article

Publication date: October 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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