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Induction and preliminary characterization of a novel halophage SNJ1 from lysogenic Natrinema sp. F5

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Abstract:

Halophage SNJ1 was induced with mitomycin C from Natrinema sp. strain F5. The phage produces plaques on Natrinema sp. strain J7 only. The phage has a head of about 67nm in diameter and a tail of 570nm in length and belongs morphologically to the family Siphoviridae. The phage is strongly salt dependent; NaCl concentration affects the integrity of SNJ1, phage adsorption, and plaque formation. The optimal NaCl concentration for phage adsorption and plaque formation is 30% and 25%, respectively.

Le halophage SNJI a été induit avec de la mitomycine C chez Natrinema sp. souche F5. Le phage produit des plaques sur la souche Natrinema sp. souche J7 seulement. Le phage possède une tête d’environ 67nm de diamètre et une queue d’une longueur de 570nm et appartient morphologiquement à la famille des Siphoviridae. Le phage est fortement dépendant du sel. La concentration de NaCl affecte l’intégrité de SNJ1, l’absorption du phage et la formation de plaques. Les concentrations optimales de NaCl à l’absorption de phage et à la formation de plaques sont de 30% et de 25% respectivement.

Document Type: Research Article

Publication date: September 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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