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Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells

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Vibrio parahaemolyticus is the leading cause of gastroenteritis from seafood consumption. We tried to determine how the gene expression levels of intestinal-like epithelial cells (Caco-2 cells) and mouse intestinal loop mucosal cells change upon infection with this bacterium. Since we found the robust production of interferon alpha (IFN-α) by the V.parahaemolyticus infection, we also assessed the upregulation of a number of IFN-stimulated genes (ISGs). The expressions of IFN protein were determined by Western blotting, and the gene expressions of Caco-2 cells after V.parahaemolyticus infection were determined by quantitative real-time reverse-transcription polymerase chain reaction. Three ISGs (i.e., IFN-α-inducible protein 15, IFN-α-inducible protein 6-16, and IFN-induced protein with tetratricopeptide repeats 1) were upregulated by V.parahaemolyticus infection. Infection induced the production of IFN-α, but not IFN- or IFN-. The upregulation of the 3 ISGs was suppressed by treatment with a neutralizing IFN-α antibody. Moreover, the production of infection-induced IFN-α was found in the mouse intestinal loop mucosal cells. V.parahaemolyticus infection of Caco-2 cells results in IFN-α production and the expression of IFN-regulated genes.

Vibrio parahaemolyticus est le principal agent responsable de la gastroentérite causée par la consommation de fruits de mer. Nous avons tenté de déterminer comment les niveaux d’expression génique d’une lignée cellulaire de type intestinal, les Caco-2, ainsi que des cellules mucosales de l’anse intestinale de souris, changent en fonction d’une infection par cette bactérie. Puisque nous avions observé une production importante d’IFN-α lors d’une infection par V.parahaemolyticus, nous avons aussi examiné la stimulation de l’expression d’un certain nombre de gènes stimulés par l’interféron (ISG). L’expression de l’IFN a été mesurée par immunobuvardage Western alors que l’expression de gènes par les Caco-2 après l’infection par V.parahaemolyticus a été mesurée par RT-PCR. L’expression de 3 ISG, la «interferon α-inducible protein-15», la «interferon α-inducible protein 6-16» et la «interferon-induced protein with tetratricopeptide repeats 1» était stimulée par l’infection par V.parahaemolyticus. L’infection a induit la production d’IFN-α contrairement aux IFN- ou IFN-. La stimulation de l’expression des 3 ISG induite par l’infection était supprimée par un traitement avec un anticorps anti-IFN-α neutralisant. La production d’IFN-α induite par l’infection a aussi été observée chez les cellules mucosales de l’anse intestinale de souris. L’infection par V.parahaemolyticus résulte en la production d’IFN-α et en l’expression de gènes régulés par l’IFN.

Document Type: Research Article

Publication date: 2007-09-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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