Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus

Authors: Innocenti, Marzia; Turchetti, Benedetta; Libkind, Diego; van Broock, Maria; Mulinacci, Nadia; Buzzini, Pietro

Source: Canadian Journal of Microbiology, Volume 53, Number 8, August 2007 , pp. 1024-1031(8)

Publisher: NRC Research Press

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Abstract:

Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography diode array detector and the main compounds were confirmed by atmospheric pressure chemical ionization quadrupole mass spectrometry. Significant (P< 0.01) differences among several species and (or) genera were observed. Thirteen strains were seen to be able to produce carotenoids, from 16.4 to 184g/g cell dry mass and from 6.0 to 1993.4g/L culture. The main carotenoids produced were identified as torularhodin, torulene, -carotene, and -carotene. The correlation matrix calculated on the basis of the carotenoid composition data matrix indicated significant (P< 0.01) relationships between torulene and torularhodin (r = 0.81), -carotene and torulene (r = 0.49), -carotene and torulene (r = –0.72), as well as -carotene and -carotene (r= 0.64). These significant correlation coefficients may suggest that species belonging to the genera Rhodosporidium, Sporobolomyces, and Sporidiobolus possess a carotenoid biosynthetic pathway analogous to that elsewhere postulated for Rhodotorula species.

Dix-huit espèces de levures du genre Rhodotorula, Rhodosporidium, Sporobolomyces et Sporidiobolus, chacune représentée par une souche type, ont été examinées afin d’évaluer leur composition en caroténoïdes. Les pigments ont été extraits des cellules, quantifiés par HPLC-DAD (high pressure liquid chromatography diode array detector) et l’identité des composés principaux a été confirmée par APCI-MS (atmospheric pressure chemical ionization quadrupole mass spectrometry). Des différences significatives ont été observées (P< 0,01) parmi les espèces et (ou) genres étudiés. Treize souches se sont révélées capables de produire des caroténoïdes, de 16,4 à 184g/g par cellule et de 6,0 à 1993,4g/L par culture, respectivement. Les principaux caroténoïdes produits ont été identifiés comme étant la torularhodine, la torulène, la -carotène et la -carotène. La matrice de corrélation calculée à partir de la matrice de données de la composition en caroténoïdes a indiqué la présence d’une relation significative (P< 0,01) entre la torulène et la torularhodine (r = 0,81), la -carotène et la torulène (r= 0,49), la -carotène et la torulène (r= –0,72) ainsi qu’entre la -carotène et la -carotène (r= 0,64). Les coefficients de corrélation significatifs présentés plus haut peuvent suggérer aussi que les espèces appartenant aux genres Rhodosporidium, Sporobolomyces et Sporidiobolus possèdent un sentier de biosynthèse des caroténoïdes analogue à celui proposé par d’autres études, chez les espèces Rhodotorula.

Document Type: Research Article

Publication date: August 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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