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Overexpression of a metacaspase gene stimulates cell growth and stress response in Schizosaccharomyces pombe

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A unique gene named pca1+, encoding a metacaspase, was cloned from the fission yeast Schizosaccharomyces pombe and was used to create a recombinant plasmid, pPMC. The metacaspase mRNA level was markedly elevated in the fission yeast cells harboring the plasmid pPMC. Overexpressed Pca1+ appeared to stimulate the growth of the fission yeast cells instead of arresting their growth. Its expression was enhanced by stress-inducing agents such as H2O2, sodium nitroprusside, and CdCl2, and it conferred cytoprotection, especially against CdCl2. However, such protection was not reproducible in the budding yeast Saccharomyces cerevisiae harboring pPMC. Taken together, these results propose that Pca1+ may be involved in the growth and stress response of the fission yeast.

Un gène unique appelé pca1+ et codant une métacaspase a été cloné chez la levure à fission Schizosaccharomyces pombe, et le plasmide recombinant pPMC a été généré. Les niveaux d’ARNm de la métacaspase étaient fortement augmentés chez les levures à fission contenant le plasmide pPMC. La surexpression de Pca1+ semblait stimuler la croissance des levures plutôt que l’arrêter. Son expression était augmentée par des agents inducteurs de stress comme le H2O2, le nitroprusside de sodium et le CdCl2, ce qui conférait une cytoprotection, particulièrement envers le CdCl2. Cependant, une telle protection ne se retrouvait pas chez la levure à bourgeonnement Saccharomyces cerevisiae contenant le pPMC. En somme, ces résultats suggèrent que Pca1+ puisse être impliqué dans la croissance et dans la réponse au stress chez la levure à fission.

Document Type: Research Article

Publication date: August 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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