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The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the pregc mutant strain of Neurospora crassa grown under phosphate starvation

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Abstract:

The preg gene encodes a cyclin-like protein that is implicated in the derepression of nucleases and phosphatases that scavenge phosphate from the environment. To better understand the regulatory role of the preg gene product, the differential display reverse transcriptase– polymerase chain reaction was used to isolate transcripts differentially expressed in the pregc mutant strain of the mold Neurospora crassa grown under phosphate starvation, at pH7.8. Two transcripts, whose differential expressions were confirmed by Northern blotting, were downregulated in a strain of N.crassa carrying a loss-of-function mutation in the preg gene (pregc allele). These transcripts revealed genes coding for enzymes involved in the thymidine salvage pathway (iso-orotate decarboxylase) and in the biosynthesis of coenzyme Q (ubiquinone C-methyltransferase), which may be relevant to a further understanding of the molecular events involved in the phosphorus sensing in N.crassa.

Le gène preg code une protéine de type cycline impliquée dans la dé-répression des nucléases et des phosphatases qui capturent le phosphate de l’environnement. Afin de mieux comprendre le rôle régulateur du produit du gène preg, une DDRT-PCR (differential display reverse transcriptase– polymerase chain reaction) a été utilisée pour isoler les transcrits exprimés de façon différentielle chez la souche mutante pregc de la moisissure Neurospora crassa cultivée en condition de privation en phosphate à pH7,8. Deux transcrits dont l’expression différentielle a été confirmée par buvardage Northern étaient régulés négativement chez une souche de N.crassa portant une mutation de perte de fonction du gène preg (allèle pregc). Ces transcrits correspondaient à des gènes codant des enzymes impliquées dans la voie de sauvegarde de la thymidine (iso-orotate décarboxylase) et de la biosynthèse de la coenzyme Q (ubiquinone C-méthyltransférase). Ces observations pourraient nous permettre de mieux comprendre les événements moléculaires impliqués de la perception du phosphore chez N.crassa.

Document Type: Research Article

Publication date: August 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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