Authors: Sebolai, Olihile M.; Pohl, Carolina H.; Botes, Piet J.; Strauss, Catharina J.; van Wyk, Pieter W.J.; Botha, Alfred; Kock, Johan L.F.

Source: Canadian Journal of Microbiology, Volume 53, Number 6, June 2007 , pp. 809-812(4)

Publisher: NRC Research Press

Abstract:

Using immunofluorescence confocal laser scanning microscopy, immunogold transmission electron microscopy and gas chromatography - mass spectrometry, we demonstrated the presence of 3-hydroxy fatty acids in Cryptococcus neoformans. Our results suggest that these oxylipins accumulate in capsules where they are released as hydrophobic droplets through tubular protuberances into the surrounding medium.

La présence d'acides gras 3-hydroxylés chez Cryptococcus neoformans a été démontrée grâce à l'utilisation de microscopie à balayage confocal au laser, à la microscopie électronique à transmission après immunomarquage à l'or et par chromatographie en phase gazeuse couplée à la spectrométrie de masse. Nos résultats suggèrent que ces oxylipines s'accumulent dans des capsules à partir desquelles elles sont libérées dans le milieu environnant sous forme de gouttelettes lipidiques à travers des protubérances tubulaires.

Document Type: Research article

Publication date: 2007-06-01

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• Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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