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Purification and characterization of Enterobacter sakazakii enterotoxin

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Abstract:

Enterobacter sakazakii has recently been recognized as an often fatal neonatal pathogen that rarely infects adults. Although not much is known about factors involved in its pathogenicity, the organism has been reported to produce enterotoxin. Currently, no information is available in the literature about the production and characterization of the enterotoxin. This report is the first attempt regarding purification and biochemical characterization of the enterotoxin produced from E.sakazakii. The toxin was purified by ammonium sulfate precipitation, followed by DEAE cellulose ion exchange and desalting by Sephadex™ G-100. The 66kDa toxin was most active at pH6 and was stable at 90°C for 30min. This stability combined with the potent activity of the toxin (LD50 = 56 pg) emphasizes the potential risk to neonates fed infant milk formula contaminated with E.sakazakii. Further detailed molecular biological studies on the toxin are warranted in view of its stability and activity.

Enterobacter sakazakii est reconnu depuis peu comme pathogène néonatal souvent fatal mais infectant rarement les adultes. Même si peu de choses sont connues quant aux facteurs impliqués dans sa pathogénicité, il semble que cet organisme produise une entérotoxine. Il n’existe actuellement aucune information dans la littérature quant à la production et la caractérisation de cette entérotoxine. Cet article décrit la première tentative de purification et de caractérisation biochimique de l’entérotoxine produite par E.sakazakii. La toxine a été purifiée par précipitation au sulfate d’ammonium, suivi par une chromatographie d’échange d’ions sur DEAE cellulose et de dessalage sur Sephadex G-100. La toxine, dont la masse moléculaire est de 66kDa, était la plus active à pH6 et était stable à 90 °C pendant 30 min. La LD50 de la toxine purifiée était de 56 pg. La très faible LD50 et la stabilité à haute température sont des indices d’un risque potentiel pour les nouveau-nés nourris au lait maternisé contaminé par E. sakazakii. Une étude plus poussée de la biologie moléculaire de cette toxine se justifie compte tenu de sa stabilité et de son activité.

Document Type: Research Article

Publication date: June 1, 2007

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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