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Metabolism of fatty acid in yeast: addition of reducing agents to the reaction medium influences -oxidation activities, -decalactone production, and cell ultrastructure in Sporidiobolus ruinenii cultivated on ricinoleic acid methyl ester

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Abstract:

The sensitivity of Sporidiobolus ruinenii yeast to the use of reducing agents, reflected in changes in the oxidoreduction potential at pH7 (Eh7) environment, ricinoleic acid methyl ester catabolism, -decalactone synthesis, cofactor level, -oxidation activity, and ultrastructure of the cell, was studied. Three environmental conditions (corresponding to oxidative, neutral, and reducing conditions) were fixed with the use of air or air and reducing agents (hydrogen and dithiothreitol). Lowering Eh7 to neutral conditions (Eh7=+30mV and+2.5 mV) favoured the production of lactone more than the more oxidative condition (Eh7=+350 mV). In contrast, when a reducing condition was used (Eh7 =–130 mV), the production of -decalactone was very low. These results were linked to changes in the cofactor ratio during lactone production, to the -oxidation activity involved in decanolide synthesis, and to ultrastructural modification of the cell.

La sensibilité de la levure Sporidiobolus ruinenii à l‘ajout de molécules réductrices a été étudiée dans des conditions de bioconversion. En particulier, la modification du Eh7 environnemental ainsi que la biotransformation de l‘acide ricinoléique en -decalactone ont été suivies. Des études sur les enzymes de la -oxydation, le niveau en cofacteurs et l‘état ultrastructurale de la cellule ont été aussi menées. Trois conditions environnementales (oxidante, neutre et réductrice) ont été obtenues par l‘utilisation d‘air seul ou en combinaison avec des molécules réductrices (hydrogène et dithiothreitol). En conditions neutres (Eh7 =+30mV et+2,5mV) la production de -decalactone est favorisée par rapport à la condition oxydante (Eh7 =+350 mV). A l‘inverse, quand une condition réductrice est appliquée (Eh7 =–130 mV), la production de -decalactone est très faible. Ces résultats ont été associés durant la production de lactone, à des modifications du ratio en cofacteurs, des activités de la -oxydation impliquées dans la synthèse de la -decalactone ainsi qu‘à des modifications ultrastructurales de la cellule.

Document Type: Research Article

Publication date: June 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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