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Myrothecium roridum Tri4 encodes a multifunctional oxygenase required for three oxygenation steps

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Abstract:

The biosyntheses of both macrocyclic trichothecenes in Myrothecium roridum and simple trichothecenes in Fusarium species begin with the cyclization of farnesyl pyrophosphate to form the sesquiterpene hydrocarbon trichodiene. A previous study showed that Myrothecium has a cluster of 3 genes that are homologous with Fusarium trichothecene genes: Tri4, a P450 oxygenase; Tri5, the sesquiterpene cyclase; and Tri6, a zinc-finger regulatory gene. Fusarium graminearum Tri4 (FgTri4) and M. roridum MrTri4 (MrTri4) have 66.9% identity. In this study, MrTri4 was expressed in Fusarium verticillioides. Liquid cultures of transformant strains expressing MrTri4 converted exogenous trichodiene to isotrichodiol, indicating that MrTri4 controls 3 oxygenation steps and that the product of MrTRI4 is isotrichodiol.

La biosynthèse de trichotécènes macrocycliques chez Myrothecium roridum et de trichothécènes simples chez Fusarium commence par la cyclisation du farnésyl pyrophosphate afin de former l’hydrocarbure de sesquiterpène trichodiène. Une étude précédente avait montré que Myrothecium possédait une grappe de 3gènes homologues aux gènes de Fusarium: Tri4, une P450 oxygénase; Tri5, une sesquiterpène cyclase; et Tri6, un gène régulateur à doigts de zinc. Les gènes Tri4 de Fusarium graminearum (FgTri4) et de M.roridum (MrTri4) possèdent 66,9% d’identité. Dans cette étude, MrTri4 a été exprimé chez Fusarium verticillioides. Des cultures liquides des souches transformantes exprimant MrTri4 convertissaient le trichodiène exogène en isotrichodiol, indiquant que MrTri4 contrôle 3 étapes d’oxygénation et que le produit de MrTRI4 est l’isotrichodiol.

Document Type: Research Article

Publication date: May 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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