Comparison of element levels in minimal and complex yeast media

Authors: Abelovska, Lenka; Bujdos, Marek; Kubova, Jana; Petrezselyova, Silvia; Nosek, Jozef; Tomaska, Lubomir

Source: Canadian Journal of Microbiology, Volume 53, Number 4, April 2007 , pp. 533-535(3)

Publisher: NRC Research Press

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Abstract:

The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown on synthetic versus complex media. In this report, we analyzed the levels of nine elements (magnesium, potassium, sodium, calcium, iron, copper, manganese, zinc, and phosphorus) and sulphate ions in commercially available peptone and yeast extract and compared them with those in yeast nitrogen base routinely used for preparation of synthetic minimal media. We observed that whereas some elements are present at similar levels, the levels of others differ by a factor as high as 20. The observed differences should be taken into account when interpreting different phenotypes observed for microbial strains grown on synthetic versus complex media.

Les fonctions cellulaires sont fortement influencées par la composition de l'environnement. En particulier, le phénotype de souches microbiennes est modulé par la concentration en ions dans les milieux de culture et des différences dans les contenus en éléments peut être responsable de la variabilité phénotypique observée lorsque les souches microbiennes sont cultivées dans du milieu synthétique comparativement à du milieu complexe. Dans ce travail, nous avons analysé les contenus en neuf éléments (magnésium, potassium, sodium, calcium, fer, cuivre, manganèse, zinc et phosphore) et en ions sulfates de peptone et d'extrait de levures disponibles commercialement et les avons comparé à ceux du yeast nitrogen base utilisé de routine dans la préparation de milieux synthétiques minimaux. Nous avons observé qu'alors que certains éléments sont présents à des niveaux similaires, le contenu en d'autres éléments diffère par un facteur aussi élevé que 20. Les différences observées devraient être considérées lors de l'interprétation de différents phénotypes observés lors de la croissance de souches microbiennes sur du milieu synthétique comparativement aux milieux complexes.

Document Type: Research article

Publication date: 2007-04-01

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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