Sequence variability in the FUM1 gene of Fusarium verticillioides strains

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Abstract:

Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%.

Les fumonisines sont des mycotoxines produites principalement par Fusarium verticillioides, lesquelles sont potentiellement cancérigènes chez l’homme et toxiques chez l’animal. La synthèse de ces toxines est dirigée par une grappe de 15 gènes où l’on retrouve FUM1, le gène le plus long qui code une polykétide synthase. Cette enzyme catalyse probablement la synthèse d’un polykétide qui forme une grande partie de la structure de la fumonisine. Dans cette étude, 27 souches possédant le gène FUM1, tel que détecté par une réaction par polymérase en chaîne, ont été analysées. Une partie du gène FUM1 a été amplifiée et séquencée à partir de 6 des 27 souches Brésiliennes isolées du maïs et du sorgho. La similarité des séquences des six souches de F. verticilloides était presque de 100 %.

Document Type: Research Article

Publication date: March 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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