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The phytopathogenic fungi Leptosphaeria maculans and Leptosphaeria biglobosa: chemotaxonomical characterization of isolates and metabolite production in different culture media

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Abstract:

Previous molecular chemotaxonomic analyses of isolates of the plant pathogenic fungus Leptosphaeria maculans (Desm.) Ces. et de Not. (asexual stage Phoma lingam (Tode ex Fr.) Desm.) in a chemically defined medium suggested that this species complex was composed of at least three distinct groups. Subsequently, a group within L. maculans was classified as Leptosphaeria biglobosa, on the basis of morphologic characteristics and the lack of sexual crossing. To obtain clarification regarding the metabolite profiles of the various groups or species of blackleg fungi, the objectives of this work were (i) to determine the chemical structures of metabolites produced by Canadian V isolates and Polish-type isolates in potato dextrose broth (PDB) and (ii) to determine the chemotaxonomic relationship among French isolates of L. biglobosa and among Canadian W isolates and Thlaspi isolates of L. maculans. Here, we report for the first time that Canadian V isolates grown in PDB produced 2,4-dihydroxy-3,6-dimethylbenzaldehyde, a metabolite never reported from L. maculans, but none of the usual phytotoxins (sirodesmins). In addition, we report a new metabolite, 2-[2-(5-hydroxybenzofuranyl)]-3-(4-hydroxyphenyl)propanenitrile, from Polish-type isolates of L. maculans grown in PDB and the metabolite profiles of 16 Thlaspi isolates. The metabolite profiles of Thlaspi isolates indicate that these are part of two distinct groups, the Polish W group and the Canadian W group, i.e., L. biglobosa. Finally, we demonstrate that the metabolite profiles of the French isolates classified as L. biglobosa are similar to those of Canadian W isolates.

De précédentes analyses moléculaires chimio-taxonomiques d’isolats du champignon phytopathogène Leptosphaeria maculans (Desm.) Ces. et de Not. (stade asexué Phoma lingam (Tode ex Fr.) Desm.) réalisées en milieu chimiquement défini ont suggéré que ce complexe d’espèces comprenait au moins trois groupes distincts. Subséquemment, un groupe trouvé au sein de L. maculans a été classifié comme étant Leptosphaeria biglobosa, selon les caractéristiques morphologiques et l’absence de croisement sexué. Afin d’obtenir des clarifications additionnelles quant aux profiles de métabolites de ces espèces ou groupes variés de champignons responsables de la maladie de la jambe noire, les objectifs de ce travail étaient : (i) de déterminer les structures chimiques des métabolites produits par les isolats Canadiens V et les isolats de type Polonais sur PDB; (ii) déterminer la relation chimio-taxonomique parmi les isolats Français de L. biglobosa, ainsi que les isolats Canadiens W et les isolats du Thlaspi de L. maculans. Nous rapportons ici pour la première fois que les isolats Canadiens V cultivés sur milieu PDB produisaient du 2,4-dihydroxy-3,6-diméthylbenzaldéhyde, un métabolite jamais encore trouvé chez L. maculans, mais aucune des phytotoxines habituelles (sirodesmines). De plus, nous rapportons la présence d’un nouveau métabolite, le 2-[2-(5-hydroxybenzofuranyl)]-3-(4-hydroxyphényl)propanenitrile, chez les isolats de L. maculans de type Polonais cultivés sur milieu PDB, ainsi que les profils métaboliques de 16 isolats du Thlaspi. Les profiles métaboliques des isolats du Thlaspi indiquent qu’ils font partie de deux groupes distincts : le groupe Polonais W et le groupe Canadien W, c’est-à-dire L. biglobosa. Finalement, nous démontrons que les profiles métaboliques des isolats Français classés chez L. biglobosa sont similaires à ceux des isolats Canadiens W.

Document Type: Research Article

Publication date: March 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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