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Importance of flagella and enterotoxins for Aeromonas virulence in a mouse model

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Abstract:

A genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaA/flaB, flaG), lateral flagella (lafA), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. When 16 Aeromonas hydrophila strains, seven Aeromonas veronii strains, and seven Aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal injection, only those strains that had one or more of the enterotoxins flaA, flaB, and either flaG or lafA showed signs of being virulent. The correlation was seen in 97% (29/30) of the strains, which included strains from drinking water. Thus, Aeromonas water isolates have the potential to be pathogenic in immunocompromised hosts.

La caractérisation génétique de huit gènes codant des facteurs de virulence soit l’élastase, la lipase, les gènes associés au flagelle polaire (flaA/flaB et flaG), le gène associé au flagelle latéral (lfaA), et les entérotoxines alt, act et ast, a été réalisée par réaction en chaîne de la polymérase dans 55 isolats d’eau de consommation et neuf isolats cliniques. Lorsque 16 souches de Aeromonas hydrophila, sept souches de Aeromonas veronii et sept souches de Aeromonas caviae présentant différentes combinaisons de facteurs de virulence ont été testées par injection intrapéritonéale dans des souris immunodéficientes, seules les souches possédant une ou plusieurs des entérotoxines flaA, flaB ou soit flaG ou lafA ont montré des signes de virulence. La corrélation a été observée chez 97 % (29/30) des souches, incluant les souches trouvées dans l’eau de consommation. Les isolats d’Aeromonas de l’eau peuvent être pathogènes chez des hôtes immuno-déficients.

Document Type: Research Article

Publication date: February 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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