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Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain

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Abstract:

Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two of them, showing both high sporulation proficiency and constitutive pseudohyphal growth, were analysed from a genetic and physiologic point of view. Each mutant carries a recessive monogenic mutation, and the two mutations reside in unlinked genes. Flocculation ability and responsiveness to different stimuli distinguished the two mutants. Growth at 37 °C affected the cell but not the colony morphology, suggesting that these two phenotypes are regulated differently. The effect of ethidium bromide, which affects mitochondrial DNA replication, suggested a possible “retrograde action” of mitochondria in pseudohyphal growth.

Saccharomyces cerevisiae est un bon modèle d’étude des effets de la différenciation morphologique sur le comportement écologique des champignons. Dans ce travail, 33 mutants morphologiques d’une souche naturelle de S. cerevisiae obtenus par mutagenèse aux UV ont été sélectionnés en fonction de la forme des stries et des cellules sur milieu enrichi. Deux d’entre eux présentant une haute compétence à la sporulation et à la croissance constitutive de pseudo-hyphes ont été analysés d’un point de vue génétique et physiologique. Chaque mutant porte une mutation récessive monogénique; les deux mutations se situent sur deux gènes non liés. La capacité de floculation et la réponse à différents stimuli permettaient de distinguer les deux mutants. La croissance à 37 °C a affecté la morphologie de la cellule mais pas celle de la colonie, suggérant que ces deux phénotypes sont régulés différemment. L’effet du bromure d’éthidium, qui affecte la réplication de l’ADN mitochondrial, suggère une possible action rétrograde de la mitochondrie sur la croissance des pseudo-hyphes.

Document Type: Research Article

Publication date: 2007-02-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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