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Bioformulation of Burkholderia sp. MSSP with a multispecies consortium for growth promotion of Cajanus cajan

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Abstract:

The present work was undertaken to formulate an effective bioformulation using Burkholderia sp. strain MSSP, a known plant-growth-promoting rhizobacterium. MSSP was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane–bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer–whey as liquid carrier. Physical and chemical properties of different low-cost carrier materials were studied. The viability of the green fluorescent tagged variant of MSSP was estimated in different sterile carrier materials. Whey and wheat bran proved to be efficient carrier materials for the bioformulation. Sawdust, rock phosphate, rice husk, and cocoa peat were average, while charcoal and sugarcane–bagasse proved to be inferior carriers. The viability of strain MSSP was also assessed in wheat bran and whey-based consortium, having three other bacterial strains, namely Sinorhizobium meliloti PP3, Rhizobium leguminosarum Pcc, and Bacillus sp. strain B1. Presence of other plant-growth-promoting bacteria did not have any detrimental effect on the viability of MSSP. Efficiency of the wheat-bran-based multispecies consortium was studied on the growth of pigeonpea in field conditions. A considerable increase in plant biomass, nodule number and weight, and number of pods was recorded as compared with individual trials and with the control.

Ce travail a été entrepris dans le but de trouver une formulation biologique efficace contenant le Burkholderia sp. MSSP, une rhizobactérie stimulant la croissance des plantes. MSSP a été étiquetée avec un gène rapporteur codant la protéine fluorescente verte (gfp) afin de suivre sa croissance sur des supports solides économiques, incluant la bagasse de sucre de canne, la sciure de bois, la tourbe de cacao, la pellicule de riz, le son de blé, le charbon, le phosphate rocheux; le petit lait de paneer étant utilisé comme véhicule liquide. Les propriétés physiques et chimiques de ces supports solides économiques ont été étudiées. La viabilité des variants MSSP étiquetés à la fluorescence a été estimée sur les différents supports solides stériles. Le petit lait et le son de blé se sont avéré d’efficaces véhicules pour une formulation biologique. La sciure de bois, le phosphate rocheux, la pellicule de riz et la tourbe de cacao étaient moyennement efficaces alors que le charbon et la bagasse de sucre de canne étaient des supports solides de qualité inférieure. La viabilité de MSSP a aussi été évaluée sur le son de blé et le petit lait en présence d’un consortium comprenant trois autres souches bactériennes : Sinorhizobium meliloti PP3, Rhizobium leguminosarum Pcc et Bacillus sp. B1. La présence d’autres bactéries favorisant la croissance des plantes n’a pas eu d’effet nuisible sur la viabilité de MSSP. L’efficacité d’un consortium multi-espèces du son de blé a été étudiée relativement à la croissance sur le terrain du pois d’Angole. Une augmentation considérable de la biomasse végétale, du nombre et du poids des nodules ainsi que du nombre de cosses a été observée, comparativement aux résultats obtenus d’essais individuels et aux groupes contrôles.

Document Type: Research Article

Publication date: February 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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