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Role of chitinase and -1,3-glucanase activities produced by a fluorescent pseudomonad and in vitro inhibition of Phytophthora capsici and Rhizoctonia solani

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Abstract:

A study was conducted to investigate the possibility of involvement of chitinase and -1,3-glucanase of an antagonistic fluorescent Pseudomonas in growth suppression of phytopathogenic fungi, Phytophthora capsici and Rhizoctonia solani. Fluorescent Pseudomonas isolates GRC3 and GRC4 were screened for their antifungal potential against phytopathogenic fungi by using dual culture technique both on solid and liquid media. The percent inhibition was calculated. Various parameters were monitored for optimization of enzyme activities by fluorescent Pseudomonas GRC3. The involvement of chitinases, -1,3-glucanases, and antifungal metabolites of nonenzymatic nature was correlated with the inhibition of P. capsici and R. solani. The results provide evidence for antibiosis as a mechanism for antagonism. The study also confirms that multiple mechanisms are involved in suppressing phytopathogens as evidenced by the involvement of chitinase and -1,3-glucanase in inhibition of R. solani but not P. capsici by isolate GRC3.

Nous avons réalisé cette étude afin d’examiner la possibilité que la chitinase et la -1,3-glucanase d’un Pseudomonas antagoniste fluorescent soient impliquées dans l’inhibition de la croissance de champignons phytopathogènes, le Phytophthora capsici et le Rhizoctonia solani. Les isolats fluorescents GRC3 et GRC4 issus de Pseudomonas ont été criblés relativement à leur potentiel antifongique envers les champignons phytopathogènes grâce à une technique de double culture en milieux liquide et solide. L’inhibition a été calculée (%). Différents paramètres d’optimization de l’activité des enzymes de Pseudomonas fluorescent GRC3 ont été examinés. L’implication de la chitinase et de la -1,3-glucanase dans l’inhibition de la croissance de P. capsici et R. solani a été évaluée. L’inhibition de la croissance de P. capsici était corrélée avec la production de métabolites antifongiques de nature non-enzymatique alors que l’inhibition de la croissance de R. solani était corrélée avec la présence de chitinase et de -1,3-glucanase. Ces résultats démontrent que l’antibiose est responsable de l’antagonisme. Cette étude confirme également que de multiples mécanismes sont impliqués dans la suppression de la croissance de phytopathogènes tel que démontré par l’implication de la chitinase et de la -1,3-glucanase dans l’inhibition de la croissance de R. solani par l’isolat GRC3, ce qui n’est pas le cas pour P. capsici.

Document Type: Research Article

Publication date: February 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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