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Antibacterial activity of aerial parts as well as in vitro raised calli of the medicinal plant Saraca asoca (Roxb.) de Wilde

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Abstract:

Leaves, stem, and flowers of Saraca asoca, an endangered medicinal plant in India, and young explants cultivated on Murashige & Skoog's medium containing 6-benzylaminopurine were analyzed for antibacterial potential. Alcoholic and aqueous extracts from parent explants and their in vitro raised calli were tested by an agar well diffusion method. Minimal inhibitory concentrations (MICs) of the extracts were determined by broth microdilution method. Aqueous extracts showed antibacterial activity against limited bacterial species, whereas alcoholic extracts were active against a wider range of bacteria. Although the alcoholic extracts of all the explants and calli showed antibacterial activity, the extracts derived from flowers and their calli showed better results. Extracts derived from calli showed comparable results to the extracts from explants. Overall, the MICs of the extracts ranged from 0.039 to 1.25 mg/mL. MICs against Gram-positive bacteria ranged from 0.078 to 1.25 mg/mL, whereas they ranged between 0.039 and 0.625 mg/mL against Gram-negative bacteria. A MIC distribution plot showed that Gram-negative bacteria were more susceptible to the extracts than Gram-positive bacteria. It is concluded that extracts of S. asoca contain antibacterial agent, and as the calli gave good results, in vitro cultivation of the explants may be used to obtain antibacterial compounds. This is the first report on antibacterial activity of S. asoca, especially through in vitro raised calli.Key words: Saraca asoca, antibacterial activity, in vitro, callus, minimal inhibitory concentration.

Les feuilles, les racines et les fleurs de Saraca asoca, une plante médicinale menacée en Inde, ainsi que de jeunes explants cultivés sur le milieu Murashige & Skoogs contenant du 6-benzylaminopurine ont été analysés relativement à leur potentiel antibactérien. Des extraits alcooliques et aqueux d'explants parentaux et de leurs cals produits in vitro ont été testés par la méthode de diffusion sur agar. Les CMI des extraits ont été déterminés par la méthode de micro-dilution. Les extraits aqueux ont montré une activité antibactérienne contre un nombre limité d'espèces alors que les extraits alcooliques étaient actifs vis-à-vis une plus grande variété de bactéries. Quoique les extraits alcooliques de tous les explants et leurs cals aient présenté une activité antibactérienne, les extraits dérivés des fleurs et de leurs cals étaient plus actifs. Les extraits dérivés des cals donnaient des résultats comparables à ceux des extraits d'explants. En somme, les CMI des extraits se situaient entre 0,039 à 1,25 mg/mL. Les CMI envers les bactéries Gram-positives se situaient entre 0,078 et 1,25 mg/mL, alors qu'elles se situaient entre 0,039 et 0,625 mg/mL envers les bactéries Gram-négatives. La courbe de distribution des CMI a montré que les bactéries Gram-négatives étaient plus sensibles aux extraits que les bactéries Gram-positives. Nous concluons ainsi que les extraits de S. asoca contiennent un agent antibactérien et que, parce que les cals donnent aussi de bons résultats, la culture in vitro des explants pourrait être utilisée pour obtenir des composés antibactériens. Ceci constitue le premier rapport de l'activité antibactérienne de S. asoca, spécialement des cals obtenues in vitro.Mots clés : Saraca asoca, activité antibactérienne, in vitro, cals, CMI.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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