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Identification of siderophores of Pseudomonas stutzeri

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Abstract:

We have identified two types of siderophores produced by Pseudomonas, one of which has never before been found in the genus. Twelve strains of Pseudomonas stutzeri belonging to genomovars 1, 2, 3, 4, 5, and 9 produced proferrioxamines, the hydroxamate-type siderophores. Pseudomonas stutzeri JM 300 (genomovar 7) and DSM 50238 (genomovar 8) and Pseudomonas balearica DSM 6082 produced amonabactins, catecholate-type siderophores. The major proferrioxamines detected were the cyclic proferrioxamines E and D2. Pseudomonas stutzeri KC also produced cyclic (X1 and X2) and linear (G1 and G2a-c) proferrioxamines. Our data indicate that the catecholate-type siderophores belong to amonabactins P 750, P 693, T 789, and T 732. A mutant of P. stutzeri KC (strain CTN1) that no longer produced the secondary siderophore pyridine-2,6-dithiocarboxylic acid continued to produce all other siderophores in its normal spectrum. Siderophore profiles suggest that strain KC (genomovar 9) belongs to the proferrioxamine-producing P. stuzeri. Moreover, a putative ferrioxamine outer membrane receptor gene foxA was identified in strain KC, and colony hybridization showed the presence of homologous receptor genes in all P. stutzeri and P. balearica strains tested.Key words: siderophore, Pseudomonas stutzeri, ferrioxamine, amonabactin.

Nous avons identifié deux types de sidérophores produits par Pseudomonas, dont un jamais encore identifié chez ce genre. Douze souches de Pseudomonas stutzeri appartenant aux génomovars 1, 2, 3, 4, 5 et 9 ont produit des proferrioxamines, des sidérophores de type hydroxamate. Pseudomonas stutzeri JM 300 (génomovar 7) et DSM 50238 (génomovar 8) ainsi que Pseudomonas balearica DSM 6082 ont produit des amonabactines, des sidérophores de type catécholate. Les principales proferrioxamines détectées étaient les proferrioxamines cycliques E et D2. Pseudomonas stutzeri KC a aussi produit des proferrioxamines cycliques (X1 et X2) et linéaires (G1 et G2a-c). Nos résultats indiquent que les sidérophores de type catécholate appartiennent aux amonabactines P 750, P 693, T 789 et T 732. Un mutant de P. stutzeri KC (la souche CTN1) qui ne produisait plus d'acide pyridine-2,6-dithiocarboxilique, un sidérophore secondaire, a continué de produire tous les autres sidérophores dans un spectre normal. Les profils de sidérophores observés suggèrent que la souche KC (génomovar 9) appartienne à P. stutzeri produisant des proferrioxamines. Qui plus est, foxA, un gène codant un récepteur présumé de ferrioxiamines présent à la surface externe, a été identifié chez la souche KC et l'hybridation de colonies a permis de démontrer la présence de gènes homologues chez toutes les souches de P. stutzeri et P. balearica testées.Mots clés : sidérophores, Pseudomonas stutzeri, ferrioxamines, amonabactine.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: December 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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