Chitinolytic bacteria in the intestinal tract of Japanese coastal fishes

Authors: Itoi, Shiro; Okamura, Toshihiro; Koyama, Yuki; Sugita, Haruo

Source: Canadian Journal of Microbiology, Volume 52, Number 12, December 2006 , pp. 1158-1163(6)

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Abstract:

Intestinal bacteria from several coastal fish species were screened on 1/20 PYBG medium containing 0.2% colloidal chitin, and 361 bacteria capable of decomposing colloidal chitin were isolated. These isolates were subsequently screened on media containing either 0.5% α-chitin or 0.5% β-chitin resulting in the identification of 31 α-chitinolytic and 275 β-chitinolytic bacterial isolates. Partial 16S rRNA gene sequencing was carried out and homology searches of the resultant sequences against the DDBJ, EMBL, and GenBank databases revealed that the majority (99%) of the chitinolytic bacteria isolated belonged to the Vibrionaceae. Phylogenetic analysis using a Bayesian approach showed that the α-chitinolytic bacteria belonging to the Vibrionaceae formed a separate cluster from the non-α-chitinolytic bacteria in the Vibrionaceae.Key words: chitinolytic bacteria, 16S rRNA, α-chitin, coastal fish, intestinal bacteria.

Les bactéries intestinales présentes dans plusieurs espèces de poissons côtiers ont été criblées sur du milieu 1/20 PYBG contenant 0,2 % de chitine colloïdale et 361 échantillons de bactéries capables de décomposer la chitine colloïdale ont été isolées. Ces isolats ont été subséquemment testés sur du milieu contenant soit 0,5 % de chitine-α ou 0,5 % de chitine-β, ce qui a permi d'identifier 31 isolats bactériens α-chitinolytiques et 275 isolats β-chitinolytiques. La séquence partielle du gène codant l'ARNr 16S a été réalisée et les recherches, dans les bases de données DDBJ, EMBL et GenBank, d'homologies avec les séquences résultantes ont révélé que la majorité (99 %) des bactéries chitinolytiques isolées appartenaient aux Vibrionacées. L'analyse phylogénique par approche bayesienne a démontré que les bactéries chitinolytiques appartenant aux Vibrionacées formaient une grappe séparée des bactéries Vibrionacées non-chitinolytiques.Mots clés : bactéries chitinolytiques, ARNr 16S, chitine-α, poissons côtiers, bactéries intestinales.

Document Type: Research article

Publication date: 2006-12-01

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Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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Chitinolytic bacteria in the intestinal tract of Japanese coastal fishes

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