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Role of the Sulfolobus shibatae viral T6 initiator in conferring promoter strength and in influencing transcription start site selection

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Abstract:

Archaeal promoters contain a TATA-box, an adjacent upstream TFB-recognition element (BRE), and a downstream initiator (INR) region from which transcription originates. While the contribution of A-box and BRE to promoter strength is well established, the role of DNA sequences within the INR region and its vicinity on transcription efficiency and start site selection remains unclear. Here, I demonstrate using the strong Sulfolobus shibatae viral T6 promoter that either substitution of its natural sequence from –17 and beyond with plasmid DNA or introduction of point transversion mutations at +3, –2, –4, and –5 positions reduce promoter strength dramatically, whereas +1, –1, and –2 mutations influence the transcription start site. These data therefore reveal that the INR region plays a role as important as the BRE and the A-box in T6 gene transcription. Key words: Archaea, transcription, initiator (INR), Sulfolobus shibatae, core promoter.

Les promoteurs archéens contiennent une boîte TATA, un élément adjacent en amont de reconnaissance TFB (BRE) et une région initiatrice (INR) en aval de laquelle la transcription débute. Bien que la contribution de la boîte A et du BRE à la force du promoteur soit bien reconnue, le rôle des séquences d'ADN à l'intérieur de la région INR et de ses environs sur l'efficacité de la transcription et sur la sélection du site d'initiation demeure obscur. Ici, je fais la démonstration en employant le promoteur viral fort T6 de Sulfolobus shibatae que la substitution de sa séquence naturelle de –17 et au-delà avec de l'ADN plasmidique ou alors l'introduction de mutations ponctuelles transversionnelles aux positions +3, –2, –4 et –5 diminuent considérablement la force du promoteur, alors que des mutations à +1, –1 et –2 influencent le site d'initiation de la transcription. Ces données révèlent par conséquent que la région INR joue un rôle aussi important que le BRE et la boîte A dans la transcription du gène T6. Mots clés : Archéens, transcription, initiateur (INR), Sulfolobus shibatae, promoteur central. [Traduit par la Rédaction]

Document Type: Research Article

Publication date: November 1, 2006

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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