Chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (Capsicum anuum L.)

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Abstract:

A climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (Capsicum anuum L.) cultivars, Milder Spiral and Ziegenhorn Bello. Structural diversity was analyzed by 16S rRNA-based terminal restriction fragment length polymorphism (T-RFLP) analysis and by the generation of 16S rRNA gene libraries to determine dominant community members in T-RFLP profiles. Cultivable community members colonizing lines Milder Spiral and Ziegenhorn Bello were identified by 16S rRNA gene analysis. T-RFLP profiles and 16S rRNA gene libraries revealed a high heterogeneity of community composition due to chilling and suggested further the existence of cultivar-specific communities. The majority of isolates obtained from the cultivar Milder Spiral were assigned as high-G+C Gram-positive bacteria (Microbacterium sp., Micrococcus sp., Rhodococcus sp.) and Firmicutes (Staphylococcus sp.). Of the isolated endophytes obtained from cultivar Zeigenhorn Bello, 93% were affiliated with Staphylococcus aureus and Bacillus sp. (Firmicutes). The experimental set-up was suited to demonstrate that chilling and cultivar type can influence the diversity of bacterial endophytes colonizing sweet pepper. We propose additional chilling experiments to investigate the effect of chilling on functional, plant-beneficial abilities of bacterial endophytes associated with low-temperature-sensitive crops, such as sweet pepper.Key words: chilling, thermophilic sweet pepper, bacterial endophyte diversity, 16S rRNA gene analysis.

Nous avons effectué une expérience en chambre climatique afin d'évaluer l'impact des basses températures (refroidissement) sur la diversité des bactéries colonisant les endosphères de deux cultivars de piments doux (Capsicum anuum L.), Milder Spiral et Ziegenhorn Bello. La diversité structurelle fut analysée par les polymorphismes de la taille des fragments de restriction terminaux basées sur l'ARNr 16S (T-RFLP) et la génération de banques de gènes d'ARNr 16S afin de déterminer les membres dominants des communautés dans les profils de T-RFLP. Les membres cultivables des communautés colonisant les lignées Milder Spiral et Zeigenhorn Bello furent identifiés par analyse de l'ARNr 16S. Les profils de T-RFLP et les banques de gènes d'ARNr 16S ont révélé une hétérogénéité élevée dans la composition des communautés attribuable au refroidissement et a de nouveau suggéré l'existence de communautés spécifiques aux cultivars. La majorité des isolats obtenus du cultivar Milder Spiral furent classifiées en tant que bactéries Gram-positives à G+C élevé (Microbacterium sp., Micrococcus sp., Rhodococcus sp.) et Firmicutes (Staphylococcus sp.). Quatre-vingt-treize % des endophytes isolés du cultivar Zeigenhorn Bello étaient affiliées à Staphylococcus aureus et Bacillus sp. (Firmicutes). Le plan expérimental a pu démontrer adéquatement que le refroidissement et le type de cultivar pouvait influencer la diversité des endophytes bactéries colonisant le piment doux. Nous proposons davantage d'expériences de refroidissement afin d'étudier l'impact du refroidissement sur les attributs fonctionnels bénéfiques aux plantes des endophytes bactériens associés avec les cultures sensibles aux basses températures telles que le piment doux.Mots clés : refroidissement, piments doux thermophiles, diversité des endophytes bactériens, analyse du gène de l'ARNr 16S.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: November 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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