Characterization and genetic variability of Fusarium verticillioides strains isolated from corn and sorghum in Brazil based on fumonisins production, microsatellites, mating type locus, and mating crosses

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Abstract:

Fusarium verticillioides (Gibberella fujikuroi mating population A) is a producer of fumonisins and one of the main contaminants of corn grains. In Brazil, some studies analyzing strains isolated from corn have demonstrated high levels of fumonisins, whereas the levels for strains isolated from sorghum have been found to be low. In the present study, we investigated the genetic diversity of 22 F. verticillioides strains isolated from corn and 21 strains isolated from sorghum cultivated in the State of São Paulo, Brazil. Differences in the genetic profile were observed between the strains isolated from the two substrates using single primer amplification reaction by polymerase chain reaction (SPAR-PCR). Fumonisins levels were higher in strains isolated from corn than in those isolated from sorghum. The MAT-1 and MAT-2 alleles were identified by PCR, and the isolates were subsequently crossed with Fusarium thapsinum (G. fujikuroi mating population F) reference strains because this species is morphologically similar to F. verticillioides and produces low levels of fumonisins. The SPAR haplotypes of some strains isolated from sorghum were similar to the F. thapsinum reference strain haplotypes, but there was no fertile mating between the strains isolated from the two substrates and the F. thapsinum references strains. The MAT-1:MAT-2 proportion was 5:17 and 14:7 for isolates from corn and sorghum, respectively.Key words: Fusarium verticillioides, fumonisins, single primer amplification reaction, mating types.

Fusarium verticillioides (population d'accouplement A de Gibberella fujikuroi) est un producteur de fumonisines et l'un des principaux contaminants des grains de maïs. Au Brésil, des études ayant analysé les souches isolées du maïs ont démontré de hauts taux de fumonisines alors que les taux étaient inférieurs chez des souches isolées du sorgho. Dans l'étude présente, nous avons examiné la diversité génétique de 22 souches de F. verticillioides isolées du maïs et 21 souches isolées du sorgho cultivé dans l'État de São Paulo, Brésil. Des différences dans les profils génétiques ont été observées entre les souches isolées des deux substrats par SPAR-PCR. Les taux de fumonisines étaient supérieurs chez les souches isolées du maïs comparativement à celles isolées du sorgho. Les allèles MAT-1 et MAT-2 ont été identifiés par PCR et les isolats furent par la suite croisés avec des souches de référence de Fusarium thapsinum (population d'accouplement F de G. fujikuroi) parce que cette espèce est morphologiquement semblable à F. verticillioides et produit de faibles niveaux de fumonisines. Les haplotypes SPAR des certaines souches isolées du sorgho étaient semblables aux haplotypes des souches des référence de F. thapsinum mais il n'y eut aucun accouplement fertile entre les souches isolées des deux substrats et les souches de référence. Les proportions MAT-1:MAT-2 des isolats du maïs et du sorgho étaient respectivement de 5:17 et de 14:7.Mots clés : Fusarium verticillioides, fumonisines, réaction d'amplification à amorces simples et types d'accouplement.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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