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Characterization of culturable bacterial populations associating with Pinus sylvestrisSuillus bovinus mycorrhizospheres

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Abstract:

Bacterial isolations were carried out on Pinus sylvestrisSuillus bovinus mycorrhizospheres obtained directly from boreal pine forest. When samples were taken during dry weather, the numbers of bacterial colony-forming units were significantly higher in uncolonized short roots and external mycelia than in mycorrhizal roots and soil outside the mycorrhizosphere. In contrast, the colony-forming unit counts were similar in all hypogeous samples after rainy weather. Culturable bacteria were absent from most Suillus bovinus sporocarps. The bacteria isolated from all types of mycorr hizo sphere samples, i.e. short roots, mycorrhizal roots, and external mycelia, consisted primarily of Burkholderia spp., whereas most isolates from soil outside the mycorrhizosphere were identified as Paenibacillus spp. This study shows that mycorrhizal external mycelia can expand the habitat favourable for common rhizosphere bacteria into the soil far from the immediate rhizosphere. Some of these bacteria may help the trees with nitrogen acquisition, since potentially diazotrophic bacteria harbouring nitrogenase reductase (nifH) genes were isolated from mycorrhizal root tips.Key words: boreal forest soil, Burkholderia, ectomycorrhiza, nitrogen fixation, Paenibacillus.

Des isolations de bactéries ont été réalisées sur des mycorhizosphères de Pinus sylvestrisSuillus bovinus obtenues directement d'une forêt de pins boréale. Lorsque les échantillons furent prélevés en temps sec, le nombre d'unités formant des colonies bactériennes était significativement supérieur dans les racines courtes non colonisées et dans les mycéliums externes comparativement aux racines mycorhiziennes et au sol à l'extérieur de la mycorhizosphère. En revanche, les comptes d'unités formant des colonies étaient semblables dans tous les échantillons hypogés après un temps pluvieux. Les bactéries cultivables étaient absentes de la plupart des sporocarpes de Suillus bovinus. Les isolats bactériens de tous les types d'échantillons de la mycorhizosphère, des racines courtes, des racines mycorhiziennes et des mycéliums externes consistaient principalement en Burkholderia spp. alors que la plupart des isolats du sol à l'extérieur de la mycorhizosphère ont été identifiés comme étant Paenibacillus spp. Cette étude démontre que les mycéliums mycorhiziens externes peuvent élargir l'habitat favorable à des bactéries communes de la rhizosphère dans le sol loin de la rhizosphère immédiate. Certaines de ces bactéries pourraient aider les arbres par l'acquisition d'azote puisque des bactéries potentiellement diazotrophes renfermant des gènes de nitrogénase réductases (nifH) ont été isolées des terminaisons racinaires mycorhiziennes.Mots clés : sol de forêt boréale, Burkholderia, ectomycorhizes, fixation de l'azote, Paenibacillus.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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