A simple and rapid method for the differentiation and identification of thermophilic bacteria

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Abstract:

In total, 170 strains of thermophilic bacteria were isolated from deep-sea hydrothermal fields in the Pacific Ocean and a hot spring in Xiamen of China. To facilitate the identification of thermophilic strains, sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins of these strains was first performed. The results showed that there exist four different protein patterns, indicating that the 170 strains might belong to four species or genera. The RAPD (random amplified polymorphic DNA) profiles of nine representative strains were consistent with those of SDS–PAGE. To further identify the species of the nine strains, their 16S rDNA sequences were analyzed. The results showed that the nine strains fell into four species of three genera, which was the same as revealed by SDS–PAGE. Therefore, SDS–PAGE of whole-cell proteins could be used as a rapid and simple method for the discrimination of thermophilic bacteria as the first step of species identification.Key words: thermophilic bacteria, SDS–PAGE, whole-cell protein, discrimination.

Un total de 170 souches de bactéries thermophiles furent isolées de champs hydrothermiques océaniques profonds dans le Pacifique et d'une source thermale à Xiamen en Chine. Pour faciliter l'identification des souches thermophiles, un électrophorèse sur gel de polyacrylamide avec dodécylsulfate de sodium (SDS–PAGE) des protéines cellulaires totales de ces souches fut tout d'abord accompli. Les résultats ont montré qu'il existait quatre différents patrons de protéines, ce qui indique que les 170 souches pourraient appartenir à quatre espèces ou genres. Les profils de RAPD (ADN polymorphe amplifié au hasard) de neuf souches représentatives étaient conformes avec ceux de SDS–PAGE. Afin d'identifier davantage les espèces des neuf souches, leurs séquences d'ADNr 16S furent analysées. Les résultats ont démontré que les neuf souches appartenaient à quatre espèces de trois genres, la même chose que ce qu'avait révélé la SDS–PAGE. Par conséquent, le SDS–PAGE de protéines cellulaires totales pourrait être utilisé comme une méthode rapide et simple pour la discrimination de bactéries thermophiles en guise de première étape vers l'identification d'espèces.Mots clés : bactéries thermophiles, SDS–PAGE, protéines cellulaires totales, discrimination.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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