Skip to main content

Characterization of a HMT2-like enzyme for sulfide oxidation from Pseudomonas putida

Buy Article:

$50.00 plus tax (Refund Policy)


The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spectra typical of a flavin adenine dinucleotide (FAD)–binding protein. The PP0053-His catalyzed a transfer of sulfide-sulfur to the thiophilic acceptor, cyanide, which decreased the Km value of the enzyme for sulfide oxidation and elevated the sulfide-dependent quinone reduction. Reaction of the enzyme with cyanide elicited a dose-dependent formation of a charge transfer band, and the FAD-cyanide adduct was supposed to work for a sulfur transfer. The pp0053 deletion from P. putida KT2440 led to activity declines of the intracellular catalase and ubiquinone-H2 oxidase. The sulfide-quinone oxidoreductase activity in P. putida KT2440 was attributable to the presence of pp0053, and the activity showed a close relevance to enzymatic activities related to sulfur assimilation.Key words: HMT2-like enzyme, pp0053, Pseudomonas putida, sulfide oxidation.

Le cadre de lecture overt pp0053 qui démontre une homologie élevée avec la séquence de la sulfure déshydrogénase mitochondriale (HMT2) accordant la tolérance au cadmium chez la levure fissipare fut amplifié à partir de Pseudomonas putida KT2440 et exprimé chez Escherichia coli JM109(DE3). La PP0053-His isolée et purifiée a démontré un spectre d'absorption typique d'une protéine se liant au FAD. La PP0053-His a catalysé le transfert de sulfure-soufre à un accepteur thiophilique, le cyanure, qui a diminué la valeur Km de l'enzyme pour l'oxydation du sulfure et a augmenté la réduction de la quinone dépendante du sulfure. La réaction de l'enzyme avec le cyanure a entraîné la formation dose dépendante d'une bande de transfert de charges et l'adduit FAD–cyanure était supposé fonctionner en faveur d'un transfert de soufre. La délétion de pp0053 de P. putida KT2440 a mené à une diminution de l'activité de la catalase intracellulaire et de l'ubiquinone-H2 oxydase. L'activité SQR chez P. putida KT2440 était attribuable à la présence de pp0053 et l'activité a démontré une forte pertinence relativement aux activités enzymatiques liées à l'assimilation du soufre.Mots clés : enzymes semblables à HMT2, pp0053, Pseudomonas putida, oxydation du sulfure.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2006

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Partial Open Access Content
Partial Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more