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Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster in Lactococcus lactis MG1363

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Abstract:

In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Tran script analysis using 5′RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1 intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster.Key words: lactic acid bacteria, acid stress, arginine catabolism.

Dans une récente analyse protéomique, nous avons montré la sur-expression des protéines ArcA et ArcB à pH acide chez Lactococcus lactis MG1363. Les gènes correspondants appartiennent au groupe arcABD1C1C2TD2 qui code les composants de la voie arginine deiminase. Dans cette étude, nous avons mieux caractériser ce cluster au niveau génétique. Des expériences d'hybridation de type Northern ont montré l'expression d'au moins sept transcrits, tous induits par l'acidité. L'analyse des transcrits par 5′RACE PCR nous a permis de caractérisé un promoteur en amont du gène arcA, et un promoteur potentiel dans la région intergénique arcB-arcD1. Les analyses in silico ont permis d'identifier neuf structures tige-boucle, toutes localisées dans les régions intergéniques. Globalement, ces résultats suggèrent un rôle de la maturation des ARNs et (ou) d'une terminaison prématurée dans l'expression différentielle des gènes du cluster arcABD1C1C2TD2.Mots clés : bactéries lactiques, stress acide, catabolisme de l'arginine.

Document Type: Research Article

Publication date: July 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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