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Optimizing the medium components in bioemulsifiers production by Candida lipolytica with response surface method

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A response surface methodology was used to study bioemulsifier production by Candida lipolytica. A 24 full experimental design was previously carried out to investigate the effects and interactions of the concentrations of corn oil, urea, ammonium sulfate, and potassium dihydrogen orthophosphate on the emulsification activity (EA) of the bioemulsifier produced by C. lipolytica. The best EA value (3.727 units of emulsification activity (UEA)) was obtained with a medium composed of 0.4 g of urea, 1.1 g of ammonium sulfate, 2.04 g of potassium dihydrogen orthophosphate, 5 mL of corn oil, 50 mL of distilled water, and 50 mL of seawater. A curvature check was performed and revealed a lack of fit of the linear approximation. The proximity of the optimum point was evident, as was the need for quadratic model and second-order designs that incorporate the effect of the curvature. Medium constituents were then optimized for the EA using a three-factor central composite design and response surface methodology. The second-order model showed statistical significance and predictive ability. It was found that the maximum EA produced was 4.415 UEA, and the optimum levels of urea, ammonium sulfate, and potassium dihydrogen orthophosphate were, respectively, 0.544% (m/v), 2.131% (m/v), and 2.628% (m/v).Key words: emulsification activity, factorial design, central composite design, optimization, biosurfactant.

Une méthodologie de surface de réponse a été employée pour étudier la production de bioemulsifier par Candida lipolytica. Des 24 pleines conceptions expérimentales précédente ont été effectuées pour étudier les effets et les interactions des concentrations d'huile de maïs, d'urée, de sulfate d'ammonium et d'orthophosphate de dihydrogène de potassium sur l'activité d'émulsification du biosurfactant produit par C. lipolytica. La meilleure valeur d'activité d'émulsification (3,727 UEA) a été obtenue avec le milieu composé par 0,4 g d'urée, de 1,1 g de sulfate d'ammonium, de 2,04 g de phosphate de potassium, de 5 mL d'huile de maïs, de 50 mL d'eau distillée et de 50 mL d'eau de mer. Le contrôle de la courbure a été effectué et manque indiqué d'ajustement de l'approximation linéaire, indiquant la proximité du point optimum et du besoin du modèle quadratique et des conceptions de second ordre qui incorporent l'effet de la courbure. Des constituants moyens ont été alors optimisés pour l'activité d'émulsification en utilisant la conception composée centrale de trois-facteur et la méthodologie extérieure de réponse. Le modèle du second degré a montré la signification statistique et les capacités prédictives. On l'a constaté que l'activité maximum d'émulsification produite était 4,415 UEA et les niveaux optima de l'urée, sulfate d'ammonium et l'orthophosphate de dihydrogène de potassium étaient respectivement, 0,544 % (m/v), 2,131 % (m/v) et 2,628 % (m/v).Mots clés : activité d'émulsification, conception factorielle, conception composée centrale, optmization, biosurfactant.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: June 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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