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Reidentification of cellulolytic enzyme-producing Trichoderma strains W-10 and G-39

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Strain W-10, originally identified as Trichoderma koningii, and its supposed mutant G-39, published for production and gene expression of cellulase and xylanase, demonstrated morphological characteristics distinct from those of T. koningii, respectively. To clarify the identification derived from morphological characteristics, several methods were used, including electrophoretic karyotyping, internal transcribed spacer (ITS) analysis of rDNA, and polymerase chain reaction (PCR) fingerprinting using the universal primer L45. All the molecular characteristics showed that strains G-39 and W-10 were identical to T. reesei and T. longibrachiatum, respectively. The results strongly supported that T. koningii G-39 and W-10 should be reassigned as T. reesei and T. longibrachiatum, respectively. Strain G-39 should be considered a mutant from T. reesei QM9414 whose spores were contaminated with those of strain W-10 during a laboratory operation. According to this, we declare that T. koningii G-39 and W-10 must be renamed as T. reesei and T. longibrachiatum, respectively.Key words: PCR fingerprinting, electrophoretic karyotypes, ITS, Trichoderma.

La souche W-10, identifiée originellement comme étant Trichoderma koningii et son supposé mutant G-39 publié pour la production et l'expression génique de cellulase et de xylanase, ont respectivement démontré des caractéristiques morphologiques distinctes de celles de T. koningii. Pour clarifier l'identification dérivée de caractéristiques morphologiques, plusieurs méthodes ont été utilisées, incluant le caryotypage électrophorétique, l'analyse des séquences internes transcrites de l'ADNr et la prise d'empreintes par réaction en chaîne de la polymérase (PCR) avec l'amorce universelle L45. Toute les caractéristiques moléculaires ont démontré que les souches G-39 et W-10 étaient identiques à des souches de T. reesei et T. longibrachiatum, respectivement. Les résultats soutiennent fortement que T. koningii G-39 et W-10 devraient être réassignés à T. reesei et T. longibrachiatum, respectivement. La souche G-39 devrait être un mutant de T. reesei QM9414 dont les spores ont été contaminées avec celles de la souche W-10 lors de travaux de laboratoire. Selon ces constatations, nous déclarons que tous les articles publiés auparavant avec T. koningii G-39 et W-10 devraient être respectivement renommés T. reesei et T. longibrachiatum.Mots clés : empreintes par PCR, caryotypage électrophorétique, ITS, Trichoderma.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: June 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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