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Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora

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Abstract:

Soft rot is a major problem encountered in potatoes during postharvest storage. The soft rot bacterium Erwinia carotovora was inhibited by a novel bacteriocin-like substance (BLS) produced by Bacillus licheniformis P40. The BLS caused a bactericidal effect on E. carotovora cells at 30 µg mL–1. Transmission electron microscopy showed that BLS-treated cells presented wrinkled bacterial surfaces and shrinkage of the whole cell, indicating plasmolysis. Erwinia carotovora cells treated with BLS were analyzed by FTIR showing differences in the 1390 cm–1 and 1250–1220 cm–1 bands, corresponding to assignments of membrane lipids. BLS was effective in preventing E. carotovora spoilage on potato tubers, reducing the symptoms of soft rot at 240 µg mL–1 and higher concentrations. Soft rot development was completely blocked at 3.7 mg mL–1. This BLS showed potential to protect potato tubers during storage. Key words: bacteriocin, plant pathogen, potato, soft rot, storage.

La pourriture molle est un problème majeur rencontré chez la pomme de terre lors de la conservation suite à la récolte. La bactérie de la pourriture molle Erwinia carotovora a été inhibée par une nouvelle substance semblable à une bactériocine (BLS) produite par la souche P40 de Bacillus licheniformis. La BLS a eu un effet bactéricide sur les cellules de E. carotovora à 30 µg mL–1. La microscopie à transmission électronique a démontré que les cellules traitées à la BLS présentaient des surfaces bactériennes plissées et un rétrécissement de la cellule entière, indiquant qu'une plasmolyse avait eu lieu. Des cellules de E. carotovora traitées avec la BLS ont été analysées par FTIR et ont démontré des différences à 1390 cm–1 et 1250–1220 cm–1, correspondant aux lipides membranaires. La BLS est efficace pour la prévention du pourrissement des tubercules de pommes de terre par E. carotovora, diminuant les symptômes de la pourriture molle à des concentrations de 240 µg mL–1 et plus. Le développement de la pourriture molle fut complètement bloqué à 3,7 mg mL–1. Cette substance démontre un potentiel pour la protection des tubercules de pommes de terre pendant la conservation. Mots clés : bactériocines, pathogènes de plantes, pommes de terre, pourriture molle, conservation. [Traduit par la Rédaction]

Document Type: Research Article

Publication date: June 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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