A spontaneous albino mutant of Ceratocystis resinifera results from a point mutation in the polyketide synthase gene, PKS1

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We characterized a spontaneous albino mutant of Ceratocystis resinifera. Compared with the wild-type progenitor strain, the albino mutant had a reduced linear growth on culture medium, but its growth on lodgepole pine sapwood was unaffected. The albino mutant did not produce any coloured pigment on agar media or wood. However, upon exposure to exogenous scytalone, an intermediate metabolite of the melanin pathway, the production of a brownish melanin was restored. This suggests that the albino phenotype resulted from a mutation affecting the melanin synthesis pathway, upstream of the scytalone synthesis step. Melanin production was restored in the mutant by transforming it with a wild-type copy of the Ceratocystis resinifera polyketide synthase gene, PKS1. The complemented transformants produced melanin, indicating that the PKS1 gene was defective in the albino mutant. Sequence analysis revealed that the PKS1 allele found in the albino contained a single point mutation that resulted in an amino acid change from serine to proline at the 3′ end of the -ketoacyl synthase motif.Key words: Ceratocystis resinifera, polyketide synthase, sap stain, albino mutant, biological control.

Nous avons caractérisé un mutant albinos spontané chez Ceratocystis resinifera. Comparativement à la souche sauvage progénitrice, le mutant albinos a une croissance linéaire réduite sur milieu nutritif gélosé, mais sa croissance dans l'aubier du pin tordu n'est pas affectée. Aucun pigment n'est produit par le mutant albinos sur milieu nutritif gélosé ou sur le bois. Toutefois, la mélanine est produite lorsque de la scytalone, un intermédiaire du sentier métabolique de la mélanine, est ajoutée au mutant. Le phénotype albinos est le produit d'une mutation dans un gène impliqué dans une étape en amont de la synthèse de la scytalone. Nous avons rétabli la production de mélanine dans le mutant albinos en le transformant avec un allèle sauvage du gène de la polycétide synthétase de Ceratocystis resinifera (PKS1). L'analyse de la séquence du PKS1 chez l'albinos révèle une mutation ponctuelle entraînant un changement d'acide aminé de sérine à proline à l'extremité 3′ du motif de -cétoacyle synthétase.Mots clés : Ceratocystis resinifera, polycétide synthétase, coloration de l'aubier, mutant albinos, biocontrôle.

Document Type: Research Article

Publication date: June 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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