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Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa

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Abstract:

Over the last few years, endophytic bacterial communities associated with citrus have been studied as key components interacting with Xylella fastidiosa. In this study, we investigated the possible interaction between the citrus endophyte Methylobacterium mesophilicum SR1.6/6 and X. fastidiosa in model plants such as Catharanthus roseus (Madagaskar periwinkle) and Nicotiana clevelandii (Clevelands tobacco). The aim of this study was to establish the fate of M. mesophilicum SR1.6/6 after inoculation of C. roseus and N. clevelandii plants, using PCR–DGGE (polymerase chain reaction – denaturing gradient gel electrophoresis) and plating techniques. Shifts in the indigenous endophytic bacterial communities were observed in plants inoculated with strain SR1.6/6, using specific primers targeting α- and -Proteobacteria. Cells of strain SR1.6/6 were observed in a biofilm structure on the root and hypocotyl surfaces of in vitro seedlings inoculated with M. mesophilicum SR1.6/6. This emphasizes the importance of these tissues as main points of entrance for this organism. The results showed that C. roseus and N. clevelandii could be used as model plants to study the interaction between M. mesophilicum and X. fastidiosa.Key words: endophytic, Methylobacterium, model plants, DGGE.

Dans les dernières années, des communautés de bactéries endophytes associées avec les agrumes ont été étudiées en tant qu'éléments clés en interaction avec Xylella fastidiosa. Dans cette étude, nous avons examiné la possibilité d'étudier l'interaction entre l'endophyte d'agrumes Methylobacterium mesophilicum souche SR1.6/6 et X. fastidiosa chez des plantes modèles comme Catharanthus roseus (petite pervenche de Madagascar) et Nicotiana clevelandii (tabac de Cleveland). Le but de cette étude fut d'établir le sort de la souche SR1.6/6 de M. mesophilicum après inoculation avec C. roseus et N. clevelandii en utilisant le PCR–DGGE (électrophorèse sur gel à gradient dénaturant) et des techniques d'ensemencement. Des changements dans les communautés bactériennes endophytes indigènes ont été observés chez les plantes inoculées avec la souche SR1.6/6 à l'aide d'amorces spécifiques ciblant les protéobactéries a et b. Les cellules de la souche SR1.6/6 ont été observées dans une structure de biofilm sur les surfaces des racines et de l'hypocotyle de semis inoculés in vitro avec la souche SR1.6/6 de M. mesophilicum. Ceci met en évidence l'importance de ces tissus comme points d'entrée principaux pour cet organisme. Les résultats montrent que C. roseus et N. clevelandii pourraient être utilisés comme plantes modèles pour l'étude de l'interaction entre M. mesophilicum et X. fastidiosa.Mots clés : endophytes, Methylobacterium, plantes modèles, DGGE.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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