Variable adhesion and diurnal population patterns of epiphytic yeasts on creeping bentgrass

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Abstract:

Irrigation and an in vitro agitation assay were used to determine the percentage of the epiphytic yeast community (Cryptococcus, Pseudozyma, Rhodotorula, and Sporobolomyces) adhering to the phylloplane of creeping bentgrass (Agrostis palustris (Huds.) Pers.). Colony-forming units (cfu) of total epiphytic yeast populations (adherent and nonadherent cells) and of adherent populations (cells not removed by agitation) were determined by leaf washing and dilution plating. In an in vitro assay, 40.0% and 57.1% of the yeast adhered to the leaves, whereas, in initial field trials the percentage of adherent yeasts ranged from 40.0% to 71.9% of the total population. Adherent yeast cfu on leaves in the morning were significantly lower on bentgrass (8.0 × 103 to 3.1 × 104 cfu·cm–2) compared with total yeast cfu (1.4 × 104 to 4.7 × 104 cfu·cm–2) on the nonirrigated control. No differences in yeast populations were observed between irrigated and nonirrigated plots 2 h after the 0900 treatments. Yeast populations followed a diurnal pattern, with larger cfu recovered from bentgrass leaves in the morning and significantly lower populations recovered in the afternoon. At 1400 the adherent yeast were 83.1%–100% of the total yeast population recovered from the leaves. The relative adhesiveness of the epiphytic yeast community on bentgrass leaves is dynamic with nonadherent cells making up a larger percentage of the population in the mornings than the afternoons.Key words: adherence, Cryptococcus, leaf surface, Rhodotorula, turfgrass.

Nous avons employé l'irrigation et un test d'agitation in vitro pour déterminer le pourcentage de la communauté de levures épiphytes (Cryptococcus, Pseudozyma, Rhodotorula, and Sporobolomyces) qui adhère au phylloplan d'agrostides traçantes (Agrostis palustris (Huds.) Pers.). Les unités formant des colonies (ufc) des populations de levures totales (cellules adhérentes et non adhérentes) et des populations adhérentes (cellules n'ayant pas été enlevées par agitation) ont été déterminées par lavage des feuilles et ensemencements de dilutions. Dans un test in vitro, 40,0 % et 57,1 % des levures adhéraient aux feuilles alors que le pourcentage de levures adhérentes oscillait entre 40,0 % et 71,9 % de la population totale dans les essais initiaux sur le terrain. Les ufc de levures adhérentes sur les feuilles d'agrostides au matin étaient significativement inférieurs (8,0 × 103 to 3,1 × 104 ufc·cm–2) comparativement aux ufc de levures totales (1,4 × 104 to 4,7 × 104 ufc·cm–2) sur le témoin non irrigué. Aucune différence dans les populations de levures n'a été observée entre les parcelles irriguées et non irriguées 2 h après les traitements de 9h00. Les populations de levures ont suivi une évolution diurne avec davantage de ufc recueillis de feuilles d'agrostides au matin et des populations significativement inférieures recueillies en après-midi. À 14h00, les levures adhérentes représentaient de 83,1 % à 100 % de la population de levures totale recueillies des feuilles. L'adhérence relative de la communauté de levures épiphytes sur des feuilles d'agrostides est dynamique, les cellules non adhérentes constituant le pourcentage majeur de la population aux matins par rapport aux après-midis.Mots clés : adhérence, Cryptococcus, surface de feuilles, Rhodotorula, gazons.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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