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Occurrence of parasporin-producing Bacillus thuringiensis in Vietnam

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Abstract:

A total of 63 Bacillus thuringiensis isolates were recovered from urban soils of Hanoi, Vietnam. Of these, 34 were identified to 12 H serogroups. None of the isolates showed larvicidal activities against three lepidopterous insects. Three isolates belonging to the two serovars, colmeri (H21) and konkukian (H34), were highly toxic to larvae of the mosquito Aedes aegypti. Parasporal inclusion proteins of four isolates exhibited cytocidal activities against HeLa cells. Immunologically, proteins of four isolates were closely related to parasporin-1 (Cry31Aa), a parasporal protein that preferentially kills human cancer cells. Haemolytic activities were associated with parasporal proteins of the three mosquitocidal isolates but not with those of the four cancer-cell-killing isolates. PCR experiments and nucleotide sequence analysis revealed that the genes of four anti-cancer isolates are closely related to the gene parasporin-1 (cry31Aa) but are dissimilar to those of the three other existing parasporins. Our results suggest that the soil of northern Vietnam is a good reservoir of parasporin-producing B. thuringiensis.Key words: Bacillus thuringiensis, soil isolate, Vietnam, mosquitocidal activity, parasporin, cancer-cell-killing activity.

Un total de 63 isolats de Bacillus thuringiensis ont été recueillis de sols urbains de Hanoi, Vietnam. De ceux-ci, 34 furent classifiées dans 12 sérogroupes H. Aucun des isolats n'a démontré d'activités larvicides contre trois insectes lépidoptères. Trois isolats appartenant aux deux sérovars colmeri (H21) et konkukian (H34), étaient fortement toxiques pour des larves du moustique Aedes aegypti. Les protéines d'inclusions parasporales de quatre isolats ont démontré des activités cytocides contre des cellules HeLa. Du point de vue immunologique, les protéines de quatre isolats étaient fortement apparentées à la parasporine-1 (Cry31Aa), une protéine parasporale qui tue de préférence les cellules cancéreuses humaines. Des activités hémolytiques furent associées avec les protéines parasporales des trois isolats tuant des moustiques mais non avec celles des quatre isolats tuant des cellules cancéreuses. Des expériences de PCR et des analyses de séquences nucléotidiques ont révélé que les gènes des trois isolats anticancéreux étaient fortement apparentés au gène parasporine-1 (cry31Aa) mais n'étaient pas semblables à ceux de trois autres parasporines existantes. Nos résultats indiquent que le sol du nord du Vietnam est un bon réservoir de B. thuringiensis produisant des parasporines.Mots clés : Bacillus thuringiensis, isolats du sol, Vietnam, activité tuant des moustiques, parasporines, activité tuant des cellules cancéreuses.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2006-04-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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