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Newly featured infection events in a supernodulating soybean mutant SS2-2 by Bradyrhizobium japonicum

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Abstract:

Supernodulating soybean (Glycine max L. Merr.) mutant SS2-2 and its wild-type counterpart, Sinpaldalkong 2, were examined for the microstructural events associated with nodule formation and development. SS2-2 produced a substantially higher percentage of curled root hairs than the wild type, especially at 14 days after inoculation with Bradyrhizobium japonicum. In addition, there was new evidence that in SS2-2, B. japonicum also entered through fissures created by the emerging adventitious root primordia. Early steps of nodule ontogeny were faster in SS2-2, and continued development of initiated nodules was more frequent and occurred at a higher frequency than in the wild type. These data suggest that the early expression of autoregulation is facilitated by decreasing the speed of cortical cell development, leading to the subsequent termination of less-developed nodules. The nodules of SS2-2 developed into spherical nodules like those formed on the wild type. In both the wild type and supernodulating mutant, vascular bundles bifurcate from root stele and branch off in the nodule cortex to surround the central infected zone. These findings indicate that SS2-2 has complete endosymbiosis and forms completely developed nodule vascular bundles like the wild type, but that the speed of nodule ontogeny differs between the wild type and SS2-2. Thus, SS2-2 has a novel symbiotic phenotype with regard to nodule organogenesis.Key words: Bradyrhizobium japonicum, early nodule development, Glycine max, root hair curling, supernodulation.

Le mutant supernodulant du soya SS2-2 et son congénère de type sauvage, Sinpaldalkong 2, ont été étudiés pour des événements microstructuraux associés avec la formation et le développement de nodules. SS2-2 a produit un pourcentage sensiblement supérieur de poils racinaires bouclés que le type sauvage, particulièrement 14 jours après l'inoculation avec Bradyrhizobium japonicum. De plus, de nouvelles preuves ont démontré que chez SS2-2, B. japonicum entrait également à travers des fissures engendrées par la racine adventive primordiale émergente. Les étapes précoces d'ontogénie nodulaires étaient plus rapides chez SS2-2 et le développement continu de nodules naissants était plus fréquent et s'est produit à une fréquence plus élevée que dans le type sauvage. Ces données suggèrent que l'expression précoce de l'autorégulation est favorisée en diminuant la vitesse de développement des cellules corticales, menant à l'élimination subséquente de nodules moins développés. Les nodules de SS2-2 se sont développés en nodules sphériques comme ceux formées sur le type sauvage. Chez les types sauvages autant que chez le mutant supernodulant, les noeuds vasculaires bifurquent de la stèle racinaire vers le cortex nodulaire pour entourer la zone centrale infectée. Ces découvertes indiquent que SS2-2 est en endosymbiose complète et forme des noeuds vasculaires de nodules complètement développés comme le type sauvage mais que la vitesse de nodulation diffère. Ainsi, SS2-2 possède un nouveau phénotype symbiotique relié à l'organogenèse des nodules.Mots clés : Bradyrhizobium japonicum, développement nodulaire précoce, Glycine max, bouclage des poils racinaires, supernodulation.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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