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Electron transport pathways for the oxidation of endogenous substrate(s) in Acidithiobacillus ferrooxidans

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Abstract:

Oxidation of endogenous substrate(s) of Acidithiobacillus ferrooxidans with O2 or Fe3+ as electron acceptor was studied in the presence of uncouplers and electron transport inhibitors. Endogenous substrate was oxidized with a respiratory quotient (CO2 produced/O2 consumed) of 1.0, indicating its carbohydrate nature. The oxidation was inhibited by complex I inhibitors (rotenone, amytal, and piericidin A) only partially, but piericidin A inhibited the oxidation with Fe3+ nearly completely. The oxidation was stimulated by uncouplers, and the stimulated activity was more sensitive to inhibition by complex I inhibitors. HQNO (2-heptyl-4-hydroxyquinoline N-oxide) also stimulated the oxidation, and the stimulated respiration was more sensitive to KCN inhibition than uncoupler stimulated respiration. Fructose, among 20 sugars and sugar alcohols including glucose and mannose, was oxidized with a CO2/O2 ratio of 1.0 by the organism. Iron chelators in general stimulated endogenous respiration, but some of them reduced Fe3+ chemically, introducing complications. The results are discussed in view of a branched electron transport system of the organism and its possible control.Key words: Acidithiobacillus ferrooxidans, endogenous respiration, uncouplers, electron transport.

L'oxydation de substrat(s) endogènes de Acidithiobacillus ferrooxidans avec O2 ou Fe3+ comme accepteurs d'électrons fut étudiée en présence de découpleurs et d'inhibiteurs du transport d'électrons. Le substrat endogène fut oxydé avec un quotient respiratoire (CO2 produit/O2 consommé) de 1,0 indiquant sa nature glucidique. L'oxydation ne fut que partiellement inhibée par des inhibiteurs du complexe I (roténone, amytal et piericidine A) mais la piericidine A a presque complètement inhibé l'oxydation avec Fe3+. L'oxydation fut stimulée par des découpleurs et l'activité stimulée était davantage sensible à l'inhibition par les inhibiteurs du complexe I. Le HQNO (2-heptyl-4-hydroxyquinoline N-oxyde) a également stimulé l'oxydation et la respiration stimulée était davantage sensible à l'inhibition par le KCN que la respiration stimulée par des découpleurs. Parmi 20 sucres et alcools de sucres incluant le glucose et le mannose, le fructose fut oxydé avec un rapport CO2/O2 de 1,0 par l'organisme. Les chélateurs de fer ont généralement stimulé la respiration endogène mais certains d'entre eux ont chimiquement réduit le Fe3+ ce qui introduisit des complications. Les résultats sont discutés dans la perspective d'un système de transport d'électrons à embranchements chez l'organisme et de sa régulation possible.Mots clés : Acidithiobacillus ferrooxidans, respiration endogène, découpleurs, transport d'électrons.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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