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Biochemical and taxonomic characterization of bacteria associated with the crucifer root maggot (Delia radicum)

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The crucifer root maggot, Delia radicum, is an important pest of cruciferous crops; however, little is known about its digestive biochemistry or resident gut microbiota. A culturing approach was used to survey the types of micro organisms associated with eggs, midgut, and faeces of larvae feeding on rutabaga. All bacteria isolated from the midgut and faecal materials were Gram-negative bacilli. Nine types of culturable bacteria were identified within the midgut based on analysis of 60 kDa chaperonin sequences and were generally -Proteobacteria, primarily Enterobacteriaceae. Carbohydrate utilization patterns, select biochemical pathways, and hydrolytic enzymes were examined using the API® system for each of the nine groups, revealing an exceptionally broad metabolic and hydrolytic potential. These studies suggest that resident alimentary tract microorganisms have the potential to contribute to host nutrition directly as a food source as well as by providing increased digestive potential.Key words: Delia radicum, crucifer root maggot, midgut-associated bacteria.

L'asticot de racines de crucifères, Delia radicum, est un important organisme nuisible des cultures de crucifères. On connaît peu de choses sur sa biochimie digestive ou sur sa microflore entérique résidante. Nous avons employé une approche basée sur la culture afin de consigner les types de micro-organismes associés avec les oeufs, l'intestin et les excréments de larves s'alimentant sur du rutabaga. Toutes les bactéries isolées de l'intestin moyen et des matières fécales étaient des bacilles Gram négatives. Neuf types de bactéries cultivables ont été identifiées à l'intérieur de l'intestin moyen selon une analyse des séquences de la chaperonine de 60 kDa et celles-ci étaient généralement des protéobactéries de la classe gamma, principalement des entérobactériacés. Les profils d'utilisation des glucides, des voies biochimiques sélectionnées et des enzymes hydrolytique ont été examinés à l'aide du système API® pour chacun des neuf groupes et ont révélé un potentiel métabolique et hydrolytique exceptionnellement large. Ces études indiquent que les micro-organismes résidants du tube alimentaire ont le potentiel de contribuer à la nutrition de l'hôte directement en tant que source alimentaire ainsi qu'en fournissant un potentiel digestif accru.Mots clés : Delia radicum, asticots de racines de crucifères, bactéries associées à l'intestin moyen.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: March 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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