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Bioreactor studies on the endophytic fungus Entrophospora infrequens for the production of an anticancer alkaloid camptothecin

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Twigs (young and old) from Nothapodytes foetida growing in the Jammu and Mahabaleshwar regions in India were used for the isolation of 52 strains of endophytic fungi and were tested for their ability to produce the anticancer alkaloid camptothecin. One of the isolates from the inner bark tissue of the N. foetida plant growing in the Jammu region of J&K state, India, was found to produce detectable quantities of camptothecin and its derivatives when grown in a semi-synthetic liquid medium. Camptothecin was identified by physicochemical analysis and further confirmed by spectroscopic studies. No camptothecin was detected in zero time cultures or in uninoculated culture broth. The maximum yield of camptothecin was 0.575 ± 0.031 mg/100 g of dry cell mass in 96 h in shake flasks, whereas 4.96 ± 0.73 mg/100 g of dry mass was recorded in 48 h in a bioreactor.Key words: endophytes, camptothecin, anticancer drug, Nothapodytes foetida, Entrophospora infrequens, bioreactor, scale-up.

Des brindilles (jeunes et vieilles) de Nothapodytes foetida poussant dans la région de Jammu et Mahabaleshwar en Inde ont été soumises à l'isolation de 52 souches de champignons endophytes et ont été analysées pour leur capacité à produire un alcaloïde anticancéreux, la camptothécine. L'un des isolats issus d'un tissu de l'écorce intérieure de la plante N. foetida poussant dans la région de Jammu dans l'état de J&K, Inde, a produit des quantités détectables de camptothécine et de ses dérivés lorsque cultivée dans un milieu liquide semi-synthétique. La camptothécine fut identifiée par analyse physico-chimique et confirmée par la suite par spectroscopie. Aucune camptothécine ne fut détectée dans des cultures au temps zéro ni dans le bouillon de culture non inoculé. Le rendement maximal de camptothécine représente une productivité de 0,575 ± 0,031 mg/100 g de masse cellulaire en 96 h dans des flacons à agitation alors que 4,96 ± 0,73 mg/100 g de masse sec fut mesuré en 48 h dans un bioréacteur.Mots clés : endophytes, camptothécine, médicament anticancéreux, Nothapodytes foetida, Entrophospora infrequens, bioréacteur, extrapolation.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2006-03-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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