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Biological control of rice bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol

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Certain plant-associated strains of fluorescent Pseudomonas spp. are known to produce the antimicrobial antibiotic 2,4-diacetylphloroglucinol (DAPG). It has antibacterial, antifungal, antiviral, and antihelminthic properties and has played a significant role in the biological control of tobacco, wheat, and sugar beet diseases. It has never been reported from India and has not been implicated in the biological suppression of a major disease of the rice crop. Here, we report that a subpopulation of 27 strains of plant-associated Pseudomonas fluorescens screened in a batch of 278 strains of fluorescent pseudomonads produced DAPG. The DAPG production was detected by a PCR-based screening method that used primers Phl2a and Phl2b and amplified a 745-bp fragment characteristic of DAPG. HPLC, 1H NMR, and IR analyses provided further evidence for its production. We report also that this compound inhibited the growth of the devastating rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae in laboratory assays and suppressed rice bacterial blight up to 59%–64% in net-house and field experiments. Tn5 mutants defective in DAPG production (Phl) of P. fluorescens PTB 9 were much less effective in their suppression of rice bacterial blight.Key words: biocontrol, 2,4-diacetylphloroglucinol, Pseudomonas fluorescens, rice, Xanthomonas oryzae pv. oryzae.

Certaines souches de Pseudomonas spp. fluorescentes associées aux plantes produisent un antibiotique antimicrobien, le 2,4-diacétylphloroglucinol (DAPG). Il possède des propriétés antibactériennes, antifongiques, antivirales et antihelminthiques, et a joué un rôle important dans la lutte biologique contre les maladies du tabac, du blé et de la betterave à sucre. On ne l'a jamais rapporté en Inde et il n'a jamais été impliqué dans la lutte biologique contre les maladies principales touchant les récoltes de riz. Nous rapportons ici qu'une sous-population de 27 souches de Pseudomonas fluorescens associées aux plantes, criblées à partir d'un lot de 278 souches de pseudomonades fluorescentes, a produit du DAPG. La production de DAPG a été détectée par une méthode de criblage par PCR à l'aide d'amorces Phl2a et Phl2b permettant d'amplifier un fragment caractéristique de 745 pb. Des analyses en HPLC, 1H NMR et IR ont fourni des évidences supplémentaires de sa production. Nous rapportons aussi que ce composé a inhibé la croissance de la rouille du riz Xanthomonas oryzae pv. oryzae, une bactérie pathogène dévastatrice, lors de tests de laboratoire, et qu'il a supprimé la rouille bactérienne de 59 % à 64 % lors de tests sur le terrain et en serres. Des mutants Tn5 de P. fluorescens PTB 9, défectifs en production de DAPG (Phl), étaient beaucoup moins efficace à supprimer la rouille bactérienne du riz.Mots clés : lutte biologique, 2,4-diacétylphloroglucinol, Pseudomonas fluorescens, riz, Xanthomonas oryzae pv. oryzae.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2006

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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