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Aureobasidium pullulans morphology: two adapted polysaccharide stains

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Morphological stages of Aureobasidium pullulans were investigated utilizing different media ingredients and were visualized by bright-field microscopy. A polysaccharide stain was developed to stain chlamydospores, cell walls, hyphae, and conidia, since current staining techniques do not reveal subcellular details to identify fungi, especially those that exhibit polysaccharide secretions.Key words: Aureobasidium, morphology, polysaccharide, pullulan, stain.

Les stades morphologiques d'Aureobasidium pullulans ont été examinés sur différents milieux de culture et observés en microscopie optique sur champ clair. Une coloration des polysaccharides a été développée afin de colorer les chlamydospores, les parois cellulaires, les hyphes et les conidies, parce que les techniques actuelles de coloration ne permettent pas d'observer les détails sub-cellulaires qui permettent d'identifier les champignons, surtout ceux qui secrètent des polysaccharides.Mots clés : Aureobasidium, morphologie, polysaccharide, pullulane, coloration.

Document Type: Research Article

Publication date: 2005-12-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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