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Cell-wall-bound metal ions are not taken up in Neurospora crassa

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To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a metal-free medium. Saturation of the surface with one metal augured subsequent dose-dependent entry of a different metal into intracellular locations. In comparison with the cobalt-resistant mutant, the cobalt-sensitive strain of N. crassa bound less cobalt on the surface but with significant intracellular accumulation. Our results demonstrate the importance of the cell wall in metal transport, toxicity, and resistance in fungi.Key words: metal transport, metal binding, cell wall, biosorption, Neurospora crassa.

Afin d'évaluer l'implication de la paroi cellulaire dans le transport des ions métalliques, nous avons examiné l'incorporation de cobalt chez Neurospora crassa. Le cobalt incorporé était surtout lié à la surface (>90 %), résultant en un relâchement de calcium et de magnésium. Le cobalt lié à la surface ne pouvait pénétrer vers les sites intracellulaires suite à une incubation subséquente des mycéliums en milieu sans métaux. La saturation de la surface avec un métal donné prédisait une entrée subséquente d'un métal différent vers les sites intracellulaires et ce, de façon dépendante de la concentration. En comparaison avec les mutants résistants au cobalt, les souches de N. crassa sensibles au cobalt liaient moins de cobalt à leur surface, mais démontraient une accumulation intracellulaire significative. Nos résultats révèlent l'importance de la membrane cellulaire dans le transport des métaux, leur toxicité et la résistance des champignons envers ceux-ci.Mots clés : transport des métaux, liaison des métaux, paroi cellulaire, bio-absorption, Neurospora crassa.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: December 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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