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Spoligotype analysis of Mycobacterium bovis isolates from Northern México

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Abstract:

Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucleotide typing (spoligotype) patterns of M. bovis isolates from cattle at Ciudad Juárez, a Mexican city close to El Paso, Texas. Fifty-eight M. bovis isolates collected from a herd in Northern Mexico were studied by spoligotyping. Nine spoligotype patterns were observed in total. Two were predominant (SB0121 and SB0140) and accounted for 50% and 14% of the isolates, respectively. Six patterns were found to be already described in an international M. bovis spoligotype database, while the other three (SB0985, SB0986, and SB0987) were novel. Interestingly, none of the isolates corresponded to any other Mexican pattern previously reported. This is the first spoligotype analysis of M. bovis strains from a border city between Mexico and the United States. The necessity for further studies to formulate a better identification of M. bovis strains within, and its dissemination between, the two countries is discussed.Key words: Mycobacterium bovis, spoligotyping, bovine tuberculosis.

La tuberculose bovine est encore fréquente en Amérique latine, entraînant des pertes économiques énormes. Il existe très peu d'études sur la dissémination de cette maladie dans cette région géographique, particulièrement aux frontières avec des pays qui sont pratiquement dépourvus de Mycobactérium bovis. Dans ces travaux, nous avons analysé les patrons de spoligotypes d'isolats de M. bovis issu de vaches de Ciudad Juárez, une ville mexicaine voisine de El Paso, Texas, USA. Cinquante-huit isolats de M. bovis recueillis d'un troupeau du nord du Mexique ont été étudiés par spoligotypage. Neuf patrons de spoligotypes ont été observés en tout, dont deux prédominants (SB0121 et SB0140) représentant respectivement 50 % et 14 % des isolats. Six patrons étaient déjà caractérisés dans une base de données internationale de spoligotypes de M. bovis, les trois autres (SB0985, SB0986 et SB0987) étant nouveaux. Fait intéressant, aucun de nos isolats n'a correspondu à un patron mexicain mentionné précédemment. Il s'agit de la première analyse de spoligotypes de souches de M. bovis de la ville frontalière entre le Mexique et les États-Unis. Nous discutons de la nécessité d'études additionnelles afin de permettre une meilleure identification des souches de M. bovis à l'intérieur des deux pays et de leur dissémination entre ceux-ci.Mots clés : Mycobactérium bovis, spoligotypage, tuberculose bovine.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: November 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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