Determination of susceptibility/resistance to antifungal drugs of Trichophyton mentagrophytes isolates by a macrodilution method

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Abstract:

Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most frequently isolated microorganism. Globally, from 3% to 10% of the human population is attacked by ony cho mycosis, and many cases involve toenails. The aim of this work was to determine the minimal inhibitory concentrations (MICs) of antifungal drugs (fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin) often used for the treatment of ungueal dermatophytosis caused by Trichophyton mentagrophytes. The MICs were determined by the broth medium macrodilution method. The results showed that activities of terbinafine and itraconazole were significantly higher (MIC <0.007–0.015 µg·mL–1 and MIC = 0.062–1.000 µg·mL–1, respectively). All isolates had reduced susceptibility to fluconazole (MIC = 16 to >64 µg·mL–1). The MICs of ketoconazole and griseofulvin varied among strains, ranging from 0.125 to 2.000 µg·mL–1 for ketoconazole and from 0.25 to 2.00 µg·mL–1 for griseofulvin. These MICs were higher than those of other studies cited, possibly because of differences in culture medium used in the other studies.Key words: dermatophytes, susceptibility testing, macrodilution, onychomycosis, antifungal drugs.

L'onychomycose est une mycose commune chez l'adulte et les dermatophytes du genre Trichophyton sont les microorganismes les plus fréquemment isolés. Globalement de 3 % à 10 % de la population humaine est atteinte d'onychomycose et plusieurs cas impliquent les ongles d'orteils. Le but de cette étude était de déterminer quelles étaient les concentrations inhibitrices minimales (CIM) de médicaments anti-fongiques (fluconazole, kétoconazole, itraconazole, terbinafine et griséofulvine) souvent utilisés pour le traitement des dermatophytoses unguéales causées Trichophyton mentagrophytes. Les CIM ont été déterminées par la méthode de macrodilution du milieu de culture. Les résultats ont montré que l'activité de la terbinafine et de l'itraconazole était significativement plus élevée (CIM <0,007–0,015 µg·mL–1 et CIM = 0,062–1,000 µg·mL–1, respectivement). Tous les isolats révélaient une susceptibilité réduite au fluconazole (CIM = 16 à >64 µg·mL–1). Les CIM du kétoconazole et de la griséofulvine variaient en fonction des souches, allant de 0,125 à 2,000 µg·mL–1 pour le kétoconazole à 0,25 à 2,00 µg·mL–1 pour la griséofulvine. Ces CIM étaient plus élevées que celles rapportées dans d'autres études citées ici, possiblement à cause de différences dans les milieux de culture utilisés dans les autres études.Mots clés : dermatophytes, test de susceptibilité, macrodilution onychomycose, médicaments anti-fongiques.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: November 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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