Effect of nitric oxide on the growth of Chlamydophila pneumoniae

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Abstract:

Chlamydophila pneumoniae is an important human intracellular pathogen; however, the pathogenesis of C. pneumoniae infection is poorly understood and the immune control mechanism versus host cells is not completely known. The role of the nitric oxide (NO) synthase pathway in inhibiting the ability of C. pneumoniae to infect macrophage J774 cells and the ability of NO to damage isolated C. pneumoniae were investigated. Exposure of infected cultures to recombinant murine gamma interferon (MurIFN-) resulted in increased production of NO and reduced viability. Addition of 2-(N,N-diethylamino)-diazenolase-2-oxide before infection of J774 cells or during chlamydial cultivation released NO, both resulting in a reduction in the viability of C. pneumoniae in a dose-dependent way. These results indicate that immune control of chlamydial growth in murine macrophage cells may trigger a mechanism that includes NO release with effects on the multiplication of the microorganism, thus suggesting that NO may play a role in preventing the systemic spread of Chlamydia.Key words: Chlamydophila pneumoniae, J774 cells, NO.

Chlamydophila pneumoniae représente un important pathogène intracellulaire humain; toutefois, la pathogenèse de l'infection par C. pneumoniae est mal comprise et le mécanisme de contrôle immunitaire par rapport aux cellules de l'hôte n'est pas complètement connu. Nous avons étudié le rôle de la voie de la synthase de l'oxyde nitrique (ON) dans l'inhibition de la capacité de C. pneumoniae à infecter les cellules macrophages J774 et la capacité de l'ON à endommager des C. pneumoniae isolées. L'exposition de cultures infectées à de l'interféron gamma recombinant murin (MurIFN-) a entraîné une production accrue de l'ON et a diminué la viabilité. L'ajout de 2-(N,N-diethylamino)-diazenolase-2-oxyde avant l'infection des cellules J774 ou pendant la culture des chlamydia a libéré de l'ON, ce qui a provoqué une diminution de la viabilité de C. pneumoniae de façon dose-dépendante. Ces résultats indiquent que le contrôle immunitaire de la croissance des chlamydia dans les cellules macrophages murines pourrait déclencher un mécanisme incluant la libération de l'ON qui aurait un impact sur la multiplication du micro-organisme, suggérant ainsi que l'ON pourrait jouer un rôle dans la prévention de la dissémination systémique de Chlamydia.Mots clés : Chlamydophila pneumoniae, cellules J774, NO.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: November 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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