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Genetic and antigenic analysis of Bordetella pertussis isolates recovered from clinical cases in Ontario, Canada, before and after the introduction of the acellular pertussis vaccine

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Sixty-eight Bordetella pertussis isolates (obtained between 1994 and 2004 from the province of Ontario in Canada) were compared by the following phenotypic and genetic analyses: serotyping; pulsed-field gel electrophoresis; and partial DNA sequence analysis of their pertactin, pertussis toxin, and fimbriae genes. Although temporal genetic variations were observed among the isolates, which is consistent with the current view that B. pertussis evolves over time, no specific antigenic or genetic type was detected in 48 isolates collected shortly after the introduction of the acellular pertussis vaccine. Further surveillance with clinical data and isolates collected periodically will be required to ensure that any genetic divergence that could affect vaccine efficacy will not be occurring.Key words: pertussis, genetic analysis, acellular vaccine.

Soixante-huit isolats de Bordetella pertussis (obtenus entre 1994 et 2004 de la province de l'Ontario au Canada) ont été comparé à l'aide des analyses phénotypiques et génétiques suivantes: sérotypage, électrophorèse sur gel en champs pulsé et séquençage partiel de l'ADN de leurs gènes de la pertactine, de la toxine pertussis et des fimbriae. Bien que des variations génétiques temporelles ont été observées parmi les isolats, ce qui s'accorde avec l'idée répandue selon laquelle B. pertussis évolue avec le temps, aucun type antigénique ou génétique spécifique ne fut détecté chez 48 isolats recueillis peu après l'introduction du vaccin anticoquelucheux acellulaire. Il sera nécessaire d'effectuer davantage de surveillance en présence de données cliniques et d'isolats recueillis périodiquement afin de s'assurer que toutes divergence génétique pouvant affecter l'efficacité du vaccin n'apparaisse.Mots clés : pertussis, analyses génétiques, vaccin acellulaire.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: September 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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