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Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale

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Growth of Aeropyrum pernix, the first reported aerobic neutrophilic hyperthermophilic archaeon, was investigated under different cultivation parameters. Different sources of seawater, pH, and the cultivation methods were tested with the aim to improve the biomass production. A 1-L glass flask fitted with a condenser and air diffuser was used as a bioreactor. The optimum conditions for maximizing A. pernix biomass were obtained when Na2S2O3·5H2O (1 g/L) with added marine broth 2216 at pH 7.0 (20 mmol HEPES buffer/L) was used as a growing medium in a 1-L flask. The biomass production was 0.45 g dry cell mass/L in 40 h under the optimum conditions, which is more than the 0.42 g dry cell mass/L in 60 h previously obtained.Key words: Aeropyrum pernix, hyperthermophilic archaeon, biomass production, simple glass flask based system.

Nous avons examiné la croissance de Aeropyrum pernix, le premier archéen aérobie neutrophile hyperthermophile, sous différents paramètres de culture. Différentes sources d'eau de mer, pH et méthodes de culture furent analysés dans le but d'améliorer la production de biomasse. Un flacon de 1 L en verre connecté à un condensateur et à un diffuseur d'air fut utilisé comme bioréacteur. Les conditions optimales permettant de maximiser la biomasse de A. pernix furent obtenues lorsque du Na2S2O3·5H2O (1 g/L) ajouté à du marine broth 2216 à un pH de 7,0 (tampon HEPES 20 mol/L) furent utilisé en tant que milieu de culture dans le flacon de 1 L. La production de biomasse fut de 0,45 g de poids cellulaire sec par litre après 40 heures dans les conditions optimales, ce qui représente davantage que les 0,42 g de poids cellulaire sec par litre après 60 heures.Mots clés : Aeropyrum pernix, archéen hyperthermophile, production de biomasse, système simple reposant sur un flacon de verre.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: September 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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